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EPS15 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EPS15 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G128252-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EPS15 (NM_001159969), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
AF-1P | AF1P | MLLT5 | Protein AF-1p | Protein Eps15 | EPS15
Origin Species
Human
NCBI GenBank
NM_001981
SwissProt
P42566

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MYLKSDSGLG GWITIPAVAD VLKYSCIVCW SSREKNNVEQ DLKEKEDTIK QRTSEVQDLQ
61DEVQRENTNL QKLQAQKQQV QELLDELDEQ KAQLEEQLKE VRKKCAEEAQ LISSLKAELT
121SQESQISTYE EELAKAREEL SRLQQETAEL EESVESGKAQ LEPLQQHLQD SQQEISSMQM
181KLMEMKDLEN HNSQLNWCSS PHSILVNGAT DYCSLSTSSS ETANLNEHVE GQSNLESEPI
241HQESPARSSP ELLPSGVTDE NEVTTAVTEK VCSELDNNRH SKEEDPFNVD SSSLTGPVAD
301TNLDFFQSDP FVGSDPFKDD PFGKIDPFGG DPFKGSDPFA SDCFFRQSTD PFATSSTDPF
361SAANNSSITS VETLKHNDPF APGGTVVAAS DSATDPFASV FGNESFGGGF ADFSTLSKVN
421NEDPFRSATS SSVSNVVITK NVFEETSVKS EDEPPALPPK IGTPTRPCPL PPGKRSINKL
481DSPDPFKLND PFQPFPGNDS PKEKDPEIFC DPFTSATTTT NKEADPSNFA NFSAYPSEED
541MIEWAKRESE REEEQRLARL NQQEQEDLEL AIALSKSEIS EA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

582 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.