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Human ENPP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human ENPP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all ENPP1 antibodies →
$220.00each
Catalog Number: LS-G78094-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human ENPP1 (NM_006208) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Alkaline phosphodiesterase I | ARHR2 | E-NPP 1 | ENPP1 | Ly-41 antigen | NPP1 | NPPS | PC1 | PDNP1 | Alkaline phosphodiesterase 1 | M6S1 | PCA1 | Pc-1
Origin Species
Human
NCBI GenBank
NM_006208
SwissProt
P22413

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.93 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDVGEEPLEK AARARTAKDP NTYKVLSLVL SVCVLTTILG CIFGLKPSCA KEVKSCKGRC
61FERTFGNCRC DAACVELGNC CLDYQETCIE PEHIWTCNKF RCGEKRLTRS LCACSDDCKD
121KGDCCINYSS VCQGEKSWVE EPCESINEPQ CPAGFETPPT LLFSLDGFRA EYLHTWGGLL
181PVISKLKKCG TYTKNMRPVY PTKTFPNHYS IVTGLYPESH GIIDNKMYDP KMNASFSLKS
241KEKFNPEWYK GEPIWVTAKY QGLKSGTFFW PGSDVEINGI FPDIYKMYNG SVPFEERILA
301VLQWLQLPKD ERPHFYTLYL EEPDSSGHSY GPVSSEVIKA LQRVDGMVGM LMDGLKELNL
361HRCLNLILIS DHGMEQGSCK KYIYLNKYLG DVKNIKVIYG PAARLRPSDV PDKYYSFNYE
421GIARNLSCRE PNQHFKPYLK HFLPKRLHFA KSDRIEPLTF YLDPQWQLAL NPSERKYCGS
481GFHGSDNVFS NMQALFVGYG PGFKHGIEAD TFENIEVYNL MCDLLNLTPA PNNGTHGSLN
541HLLKNPVYTP KHPKEVHPLV QCPFTRNPRD NLGCSCNPSI LPIEDFQTQF NLTVAEEKII
601KHETLPYGRP RVLQKENTIC LLSQHQFMSG YSQDILMPLW TSYTVDRNDS FSTEDFSNCL
661YQDFRIPLSP VHKCSFYKNN TKVSYGFLSP PQLNKNSSGI YSEALLTTNI VPMYQSFQVI
721WRYFHDTLLR KYAEERNGVN VVSGPVFDFD YDGRCDSLEN LRQKRRVIRN QEILIPTHFF
781IVLTSCKDTS QTPLHCENLD TLAFILPHRT DNSESCVHGK HDSSWVEELL MLHRARITDV
841EHITGLSFYQ QRKEPVSDIL KLKTHLPTFS QED
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

873 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.