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ENPP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

ENPP1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G129143-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human ENPP1 (NM_006208), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Alkaline phosphodiesterase I | ARHR2 | E-NPP 1 | ENPP1 | Ly-41 antigen | NPP1 | NPPS | PC1 | PDNP1 | Alkaline phosphodiesterase 1 | M6S1 | PCA1 | Pc-1
Origin Species
Human
NCBI GenBank
NM_006208
SwissProt
P22413

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
99.93 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDVGEEPLEK AARARTAKDP NTYKVLSLVL SVCVLTTILG CIFGLKPSCA KEVKSCKGRC
61FERTFGNCRC DAACVELGNC CLDYQETCIE PEHIWTCNKF RCGEKRLTRS LCACSDDCKD
121KGDCCINYSS VCQGEKSWVE EPCESINEPQ CPAGFETPPT LLFSLDGFRA EYLHTWGGLL
181PVISKLKKCG TYTKNMRPVY PTKTFPNHYS IVTGLYPESH GIIDNKMYDP KMNASFSLKS
241KEKFNPEWYK GEPIWVTAKY QGLKSGTFFW PGSDVEINGI FPDIYKMYNG SVPFEERILA
301VLQWLQLPKD ERPHFYTLYL EEPDSSGHSY GPVSSEVIKA LQRVDGMVGM LMDGLKELNL
361HRCLNLILIS DHGMEQGSCK KYIYLNKYLG DVKNIKVIYG PAARLRPSDV PDKYYSFNYE
421GIARNLSCRE PNQHFKPYLK HFLPKRLHFA KSDRIEPLTF YLDPQWQLAL NPSERKYCGS
481GFHGSDNVFS NMQALFVGYG PGFKHGIEAD TFENIEVYNL MCDLLNLTPA PNNGTHGSLN
541HLLKNPVYTP KHPKEVHPLV QCPFTRNPRD NLGCSCNPSI LPIEDFQTQF NLTVAEEKII
601KHETLPYGRP RVLQKENTIC LLSQHQFMSG YSQDILMPLW TSYTVDRNDS FSTEDFSNCL
661YQDFRIPLSP VHKCSFYKNN TKVSYGFLSP PQLNKNSSGI YSEALLTTNI VPMYQSFQVI
721WRYFHDTLLR KYAEERNGVN VVSGPVFDFD YDGRCDSLEN LRQKRRVIRN QEILIPTHFF
781IVLTSCKDTS QTPLHCENLD TLAFILPHRT DNSESCVHGK HDSSWVEELL MLHRARITDV
841EHITGLSFYQ QRKEPVSDIL KLKTHLPTFS QED
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

873 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.