EEF1D Antibody
Catalog Number: LS-C341419
2 sizes / from $364.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human EEF1D Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G75703-20
Catalog Number: LS-G75703
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human EEF1D (NM_032378) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Antigen NY-CO-4 | EEF1D | EF-1D | EF-1-delta | Elongation factor 1-delta | FP1047 | EF1D
- Origin Species
- Human
- NCBI GenBank
- NM_032378
- SwissProt
- P29692
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 71.2 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRSGKASCTL ETVWEDKHKY EEAERRFYEH EATQAAASAQ QLPAEGPAMN GPGQDDPEDA61DEAEAPDGGS RRDPRKSQDS RKPLQKKRKR SPKSGLGPAD LALLGLSAER VWLDKSLFDQ121AESSYRQKLA DVAAQAAWPP ALAPWGLCTH GNQVACHHVT WGIWVNKSSF DQAERAFVEW181SQALLLAPEG SRRQGTPNTG QQVAVPDLAH QPSPPVNGQP PLGSLQALVR EVWLEKPRYD241AAERGFYEAL FDGHPPGKVR LQERAGLAEG ARRGRRDRRG RNILGNKRAG LRRADGEAPS301ALPYCYFLQK DAEAPWLSKP AYDSAECRHH AAEALRVAWC LEAASLSHRP GPRSGLSVSS361LRPNRKMATN FLAHEKIWFD KFKYDDAERR FYEQMNGPVA GASRQENGAS VILRDIARAR421ENIQKSLAGS SGPGASSGTS GDHGELVVRI ASLEVENQSL RGVVQELQQA ISKLEARLNV481LEKSSPGHRA TAPQTQHVSP MRQVEPPAKK PATPAEDDED DDIDLFGSDN EEEDKEAAQL541REERLRQYAE KKAKKPALVA KSSILLDVKP WDDETDMAQL EACVRSIQLD GLVWGASKLV601PVGYGIRKLQ IQCVVEDDKV GTDLLEEEIT KFEEHVQSVD IAAFNKISGPSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
650 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
