LSBio
EEF1D Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

EEF1D Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all EEF1D antibodies →
$504.00each
Catalog Number: LS-G118275-100

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human EEF1D (NM_001960), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Antigen NY-CO-4 | EEF1D | EF-1D | EF-1-delta | Elongation factor 1-delta | FP1047 | EF1D
Origin Species
Human
NCBI GenBank
NM_032378
SwissProt
P29692

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
71.42 kDa
Protein Type
Lysate
Amino Acid Sequence
1MRSGKASCTL ETVWEDKHKY EEAERRFYEH EATQAAASAQ QLPAEGPAMN GPGQDDPEDA
61DEAEAPDGGS RRDPRKSQDS RKPLQKKRKR SPKSGLGPAD LALLGLSAER VWLDKSLFDQ
121AESSYRQKLA DVAAQAAWPP ALAPWGLCTH GNQVACHHVT WGIWVNKSSF DQAERAFVEW
181SQALLLAPEG SRRQGTPNTG QQVAVPDLAH QPSPPVNGQP PLGSLQALVR EVWLEKPRYD
241AAERGFYEAL FDGHPPGKVR LQERAGLAEG ARRGRRDRRG RNILGNKRAG LRRADGEAPS
301ALPYCYFLQK DAEAPWLSKP AYDSAECRHH AAEALRVAWC LEAASLSHRP GPRSGLSVSS
361LRPNRKMATN FLAHEKIWFD KFKYDDAERR FYEQMNGPVA GASRQENGAS VILRDIARAR
421ENIQKSLAGS SGPGASSGTS GDHGELVVRI ASLEVENQSL RGVVQELQQA ISKLEARLNV
481LEKSSPGHRA TAPQTQHVSP MRQVEPPAKK PATPAEDDED DDIDLFGSDN EEEDKEAAQL
541REERLRQYAE KKAKKPALVA KSSILLDVKP WDDETDMAQL EACVRSIQLD GLVWGASKLV
601PVGYGIRKLQ IQCVVEDDKV GTDLLEEEIT KFEEHVQSVD IAAFNKI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

647 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.