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Human E6TP1 / SIPA1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human E6TP1 / SIPA1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G79887-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SIPA1L1 (NM_015556) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
E6-targeted protein 1 | H_DJ1140G11.1 | KIAA0440 | SIPA1-like protein 1 | SIPA1L1 | E6TP1 | WUGSC:H_DJ1140G11.1
Origin Species
Human
NCBI GenBank
NM_015556
SwissProt
O43166

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
199.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MTSLKRSQTE RPLATDRASV VGTDGTPKVH TDDFYMRRFR SQNGSLGSSV MAPVGPPRSE
61GSHHITSTPG VPKMGVRARI ADWPPRKENI KESSRSSQEI ETSSCLDSLS SKSSPVSQGS
121SVSLNSNDSA MLKSIQNTLK NKTRPSENMD SRFLMPEAYP SSPRKALRRI RQRSNSDITI
181SELDVDSFDE CISPTYKTGP SLHREYGSTS SIDKQGTSGE SFFDLLKGYK DDKSDRGPTP
241TKLSDFLITG GGKGSGFSLD VIDGPISQRE NLRLFKEREK PLKRRSKSET GDSSIFRKLR
301NAKGEELGKS SDLEDNRSED SVRPWTCPKC FAHYDVQSIL FDLNEAIMNR HNVIKRRNTT
361TGASAAAVAS LVSGPLSHSA SFSSPMGSTE DLNSKGSLSM DQGDDKSNEL VMSCPYFRNE
421IGGEGERKIS LSKSNSGSFS GCESASFEST LSSHCTNAGV AVLEVPKENL VLHLDRVKRY
481IVEHVDLGAY YYRKFFYQKE HWNYFGADEN LGPVAVSIRR EKPDEMKENG SPYNYRIIFR
541TSELMTLRGS VLEDAIPSTA KHSTARGLPL KEVLEHVVPE LNVQCLRLAF NTPKVTEQLM
601KLDEQGLNYQ QKVGIMYCKA GQSTEEEMYN NESAGPAFEE FLQLLGERVR LKGFEKYRAQ
661LDTKTDSTGT HSLYTTYKDY EIMFHVSTML PYTPNNKQQL LRKRHIGNDI VTIVFQEPGA
721QPFSPKNIRS HFQHVFVIVR VHNPCSDSVC YSVAVTRSRD VPSFGPPIPK GVTFPKSNVF
781RDFLLAKVIN AENAAHKSEK FRAMATRTRQ EYLKDLAEKN VTNTPIDPSG KFPFISLASK
841KKEKSKPYPG AELSSMGAIV WAVRAEDYNK AMELDCLLGI SNEFIVLIEQ ETKSVVFNCS
901CRDVIGWTST DTSLKIFYER GECVSVGSFI NIEEIKEIVK RLQFVSKGCE SVEMTLRRNG
961LGQLGFHVNY EGIVADVEPY GYAWQAGLRQ GSRLVEICKV AVATLSHEQM IDLLRTSVTV
1021KVVIIPPHDD CTPRRSCSET YRMPVMEYKM NEGVSYEFKF PFRNNNKWQR NASKGPHSPQ
1081VPSQVQSPMT SRLNAGKGDG KMPPPERAAN IPRSISSDGR PLERRLSPGS DIYVTVSSMA
1141LARSQCRNSP SNLSSSSDTG SVGGTYRQKS MPEGFGVSRR SPASIDRQNT QSDIGGSGKS
1201TPSWQRSEDS IADQMAYSYR GPQDFNSFVL EQHEYTEPTC HLPAVSKVLP AFRESPSGRL
1261MRQDPVVHLS PNKQGHSDSH YSSHSSSNTL SSNASSAHSD EKWYDGDRTE SELNSYNYLQ
1321GTSADSGIDT TSYGPSHGST ASLGAATSSP RSGPGKEKVA PLWHSSSEVI SMADRTLETE
1381SHGLDRKTES SLSLDIHSKS QAGSTPLTRE NSTFSINDAA SHTSTMSSRH SASPVVFTSA
1441RSSPKEELHP AAPSQLAPSF SSSSSSSSGP RSFYPRQGAT SKYLIGWKKP EGTINSVGFM
1501DTRKRHQSDG NEIAHTRLRA STRDLRASPK PTSKSTIEED LKKLIDLESP TPESQKSFKF
1561HALSSPQSPF PSTPTSRRAL HRTLSDESIY NSQREHFFTS RASLLDQALP NDVLFSSTYP
1621SLPKSLPLRR PSYTLGMKSL HGEFSASDSS LTDIQETRRQ PMPDPGLMPL PDTAADLDWS
1681NLVDAAKAYE VQRASFFAAS DENHRPLSAA SNSDQLEDQA LAQMKPYSSS KDSSPTLASK
1741VDQLEGMLKM LREDLKKEKE DKAHLQAEVQ HLREDNLRLQ EESQNASDKL KKFTEWVFNT
1801IDMS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1804 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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