Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
E6TP1 / SIPA1L1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113141-100
Catalog Number: LS-G113141
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SIPA1L1 (NM_015556), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- E6-targeted protein 1 | H_DJ1140G11.1 | KIAA0440 | SIPA1-like protein 1 | SIPA1L1 | E6TP1 | WUGSC:H_DJ1140G11.1
- Origin Species
- Human
- NCBI GenBank
- NM_015556
- SwissProt
- O43166
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 199.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MTSLKRSQTE RPLATDRASV VGTDGTPKVH TDDFYMRRFR SQNGSLGSSV MAPVGPPRSE61GSHHITSTPG VPKMGVRARI ADWPPRKENI KESSRSSQEI ETSSCLDSLS SKSSPVSQGS121SVSLNSNDSA MLKSIQNTLK NKTRPSENMD SRFLMPEAYP SSPRKALRRI RQRSNSDITI181SELDVDSFDE CISPTYKTGP SLHREYGSTS SIDKQGTSGE SFFDLLKGYK DDKSDRGPTP241TKLSDFLITG GGKGSGFSLD VIDGPISQRE NLRLFKEREK PLKRRSKSET GDSSIFRKLR301NAKGEELGKS SDLEDNRSED SVRPWTCPKC FAHYDVQSIL FDLNEAIMNR HNVIKRRNTT361TGASAAAVAS LVSGPLSHSA SFSSPMGSTE DLNSKGSLSM DQGDDKSNEL VMSCPYFRNE421IGGEGERKIS LSKSNSGSFS GCESASFEST LSSHCTNAGV AVLEVPKENL VLHLDRVKRY481IVEHVDLGAY YYRKFFYQKE HWNYFGADEN LGPVAVSIRR EKPDEMKENG SPYNYRIIFR541TSELMTLRGS VLEDAIPSTA KHSTARGLPL KEVLEHVVPE LNVQCLRLAF NTPKVTEQLM601KLDEQGLNYQ QKVGIMYCKA GQSTEEEMYN NESAGPAFEE FLQLLGERVR LKGFEKYRAQ661LDTKTDSTGT HSLYTTYKDY EIMFHVSTML PYTPNNKQQL LRKRHIGNDI VTIVFQEPGA721QPFSPKNIRS HFQHVFVIVR VHNPCSDSVC YSVAVTRSRD VPSFGPPIPK GVTFPKSNVF781RDFLLAKVIN AENAAHKSEK FRAMATRTRQ EYLKDLAEKN VTNTPIDPSG KFPFISLASK841KKEKSKPYPG AELSSMGAIV WAVRAEDYNK AMELDCLLGI SNEFIVLIEQ ETKSVVFNCS901CRDVIGWTST DTSLKIFYER GECVSVGSFI NIEEIKEIVK RLQFVSKGCE SVEMTLRRNG961LGQLGFHVNY EGIVADVEPY GYAWQAGLRQ GSRLVEICKV AVATLSHEQM IDLLRTSVTV1021KVVIIPPHDD CTPRRSCSET YRMPVMEYKM NEGVSYEFKF PFRNNNKWQR NASKGPHSPQ1081VPSQVQSPMT SRLNAGKGDG KMPPPERAAN IPRSISSDGR PLERRLSPGS DIYVTVSSMA1141LARSQCRNSP SNLSSSSDTG SVGGTYRQKS MPEGFGVSRR SPASIDRQNT QSDIGGSGKS1201TPSWQRSEDS IADQMAYSYR GPQDFNSFVL EQHEYTEPTC HLPAVSKVLP AFRESPSGRL1261MRQDPVVHLS PNKQGHSDSH YSSHSSSNTL SSNASSAHSD EKWYDGDRTE SELNSYNYLQ1321GTSADSGIDT TSYGPSHGST ASLGAATSSP RSGPGKEKVA PLWHSSSEVI SMADRTLETE1381SHGLDRKTES SLSLDIHSKS QAGSTPLTRE NSTFSINDAA SHTSTMSSRH SASPVVFTSA1441RSSPKEELHP AAPSQLAPSF SSSSSSSSGP RSFYPRQGAT SKYLIGWKKP EGTINSVGFM1501DTRKRHQSDG NEIAHTRLRA STRDLRASPK PTSKSTIEED LKKLIDLESP TPESQKSFKF1561HALSSPQSPF PSTPTSRRAL HRTLSDESIY NSQREHFFTS RASLLDQALP NDVLFSSTYP1621SLPKSLPLRR PSYTLGMKSL HGEFSASDSS LTDIQETRRQ PMPDPGLMPL PDTAADLDWS1681NLVDAAKAYE VQRASFFAAS DENHRPLSAA SNSDQLEDQA LAQMKPYSSS KDSSPTLASK1741VDQLEGMLKM LREDLKKEKE DKAHLQAEVQ HLREDNLRLQ EESQNASDKL KKFTEWVFNT1801IDMSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1804 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.