DUSP27 Protein (Over-Expression Lysate Myc-DDK (Flag))
Catalog Number: LS-G120212-100
100 µg (1 mg/mL) / $725.00
Expression System Human Embryonic Kidney 293
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DUSP27 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83623-20
Catalog Number: LS-G83623
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DUSP27 (NM_001080426) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DUSP27
- Origin Species
- Human
- NCBI GenBank
- AF119045
- SwissProt
- Q5VZP5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 130 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MATRKDTEEE QVVPSEEDEA NVRAVQAHYL RSPSPSQYSM VSDAETESIF MEPIHLSSAI61AAKQIINEEL KPPGVRADAE CPGMLESAEQ LLVEDLYNRV REKMDDTSLY NTPCVLDLQR121ALVQDRQEAP WNEVDEVWPN VFIAEKSVAV NKGRLKRLGI THILNAAHGT GVYTGPEFYT181GLEIQYLGVE VDDFPEVDIS QHFRKASEFL DEALLTYRGK VLVSSEMGIS RSAVLVVAYL241MIFHNMAILE ALMTVRKKRA IYPNEGFLKQ LRELNEKLME EREEDYGREG GSAEAEEGEG301TGSMLGARVH ALTVEEEDDS ASHLSGSSLG KATQASKPLT LIDEEEEEKL YEQWKKGQGL361LSDKVPQDGG GWRSASSGQG GEELEDEDVE RIIQEWQSRN ERYQAEGYRR WGREEEKEEE421SDAGSSVGRR RRTLSESSAW ESVSSHDIWV LKQQLELNRP DHGRRRRADS MSSESTWDAW481NERLLEIEKE ASRRYHAKSK REEAADRSSE AGSRVREDDE DSVGSEASSF YNFCSRNKDK541LTALERWKIK RIQFGFHKKD LGAGDSSGEP GAEEAVGEKN PSDVSLTAYQ AWKLKHQKKV601GSENKEEVVE LSKGEDSALA KKRQRRLELL ERSRQTLEES QSMASWEADS STASGSIPLS661AFWSADPSVS ADGDTTSVLS TQSHRSHLSQ AASNIAGCST SNPTTPLPNL PVGPGDTISI721ASIQNWIANV VSETLAQKQN EMLLLSRSPS VASMKAVPAA SCLGDDQVSM LSGHSSSSLG781GCLLPQSQAR PSSDMQSVLS CNTTLSSPAE SCRSKVRGTS KPIFSLFADN VDLKELGRKE841KEMQMELREK MSEYKMEKLA SDNKRSSLFK KKKVKEDEDD GVGDGDEDTD SAIGSFRYSS901RSNSQKPETD TCSSLAVCDH YASGSRVGKE MDSSINKWLS GLRTEEKPPF QSDWSGSSRG961KYTRSSLLRE TESKSSSYKF SKSQSEEQDT SSYHEANGNS VRSTSRFSSS STREGREMHK1021FSRSTYNETS SSREESPEPY FFRRTPESSE REESPEPQRP NWARSRDWED VEESSKSDFS1081EFGAKRKFTQ SFMRSEEEGE KERTENREEG RFASGRRSQY RRSTDREEEE EMDDEAIIAA1141WRRRQEETRT KLQKRREDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1158 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
