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DUSP27 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DUSP27 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DUSP27 antibodies →
$725.00each
Catalog Number: LS-G120212-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DUSP27 (NM_001080426), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DUSP27
Origin Species
Human
NCBI GenBank
AF119045
SwissProt
Q5VZP5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
130 kDa
Protein Type
Lysate
Amino Acid Sequence
1MATRKDTEEE QVVPSEEDEA NVRAVQAHYL RSPSPSQYSM VSDAETESIF MEPIHLSSAI
61AAKQIINEEL KPPGVRADAE CPGMLESAEQ LLVEDLYNRV REKMDDTSLY NTPCVLDLQR
121ALVQDRQEAP WNEVDEVWPN VFIAEKSVAV NKGRLKRLGI THILNAAHGT GVYTGPEFYT
181GLEIQYLGVE VDDFPEVDIS QHFRKASEFL DEALLTYRGK VLVSSEMGIS RSAVLVVAYL
241MIFHNMAILE ALMTVRKKRA IYPNEGFLKQ LRELNEKLME EREEDYGREG GSAEAEEGEG
301TGSMLGARVH ALTVEEEDDS ASHLSGSSLG KATQASKPLT LIDEEEEEKL YEQWKKGQGL
361LSDKVPQDGG GWRSASSGQG GEELEDEDVE RIIQEWQSRN ERYQAEGYRR WGREEEKEEE
421SDAGSSVGRR RRTLSESSAW ESVSSHDIWV LKQQLELNRP DHGRRRRADS MSSESTWDAW
481NERLLEIEKE ASRRYHAKSK REEAADRSSE AGSRVREDDE DSVGSEASSF YNFCSRNKDK
541LTALERWKIK RIQFGFHKKD LGAGDSSGEP GAEEAVGEKN PSDVSLTAYQ AWKLKHQKKV
601GSENKEEVVE LSKGEDSALA KKRQRRLELL ERSRQTLEES QSMASWEADS STASGSIPLS
661AFWSADPSVS ADGDTTSVLS TQSHRSHLSQ AASNIAGCST SNPTTPLPNL PVGPGDTISI
721ASIQNWIANV VSETLAQKQN EMLLLSRSPS VASMKAVPAA SCLGDDQVSM LSGHSSSSLG
781GCLLPQSQAR PSSDMQSVLS CNTTLSSPAE SCRSKVRGTS KPIFSLFADN VDLKELGRKE
841KEMQMELREK MSEYKMEKLA SDNKRSSLFK KKKVKEDEDD GVGDGDEDTD SAIGSFRYSS
901RSNSQKPETD TCSSLAVCDH YASGSRVGKE MDSSINKWLS GLRTEEKPPF QSDWSGSSRG
961KYTRSSLLRE TESKSSSYKF SKSQSEEQDT SSYHEANGNS VRSTSRFSSS STREGREMHK
1021FSRSTYNETS SSREESPEPY FFRRTPESSE REESPEPQRP NWARSRDWED VEESSKSDFS
1081EFGAKRKFTQ SFMRSEEEGE KERTENREEG RFASGRRSQY RRSTDREEEE EMDDEAIIAA
1141WRRRQEETRT KLQKRRED
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1158 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.