DP97 / DDX54 Antibody
Catalog Number: LS-C804835
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DP97 / DDX54 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83892-20
Catalog Number: LS-G83892
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DDX54 (NM_001111322) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APR-5 | DEAD box protein 54 | DDX54 | DEAD box helicase 97 KDa | DP97 | DEAD box RNA helicase 97 kDa
- Origin Species
- Human
- NCBI GenBank
- NM_024072
- SwissProt
- Q8TDD1
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA EDDARARKLG61PGRPLPTFPT SECTSDVEPD TREMVRAQNK KKKKSGGFQS MGLSYPVFKG IMKKGYKVPT121PIQRKTIPVI LDGKDVVAMA RTGSGKTACF LLPMFERLKT HSAQTGARAL ILSPTRELAL181QTLKFTKELG KFTGLKTALI LGGDRMEDQF AALHENPDII IATPGRLVHV AVEMSLKLQS241VEYVVFDEAD RLFEMGFAEQ LQEIIARLPG GHQTVLFSAT LPKLLVEFAR AGLTEPVLIR301LDVDTKLNEQ LKTSFFLVRE DTKAAVLLHL LHNVVRPQDQ TVVFVATKHH AEYLTELLTT361QRVSCAHIYS ALDPTARKIN LAKFTLGKCS TLIVTDLAAR GLDIPLLDNV INYSFPAKGK421LFLHRVGRVA RAGRSGTAYS LVAPDEIPYL LDLHLFLGRS LTLARPLKEP SGVAGVDGML481GRVPQSVVDE EDSGLQSTLE ASLELRGLAR VADNAQQQYV RSRPAPSPES IKRAKEMDLV541GLGLHPLFSS RFEEEELQRL RLVDSIKNYR SRATIFEINA SSRDLCSQVM RAKRQKDRKA601IARFQQGQQG RQEQQEGPVG PAPSRPALQE KQPEKEEEEE AGESVEDIFS EVVGRKRQRS661GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE GGAFEQQAAG AVLDLMGDEA721QNLTRGRQQL KWDRKKKRFV GQSGQEDKKK IKTESGRYIS SSYKRDLYQK WKQKQKIDDR781DSDEEGASDR RGPERRGGKR DRGQAGASRP HAPGTPAGRV RPELKTKQQI LKQRRRAQKL841HFLQRGGLKQ LSARNRRRVQ ELQQGAFGRG ARSKKGKMRK RMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
882 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
