Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DP97 / DDX54 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G124241-100
Catalog Number: LS-G124241
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX54 (NM_001111322), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- APR-5 | DEAD box protein 54 | DDX54 | DEAD box helicase 97 KDa | DP97 | DEAD box RNA helicase 97 kDa
- Origin Species
- Human
- NCBI GenBank
- NM_024072
- SwissProt
- Q8TDD1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 98.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAADKGPAAG PRSRAAMAQW RKKKGLRKRR GAASQARGSD SEDGEFEIQA EDDARARKLG61PGRPLPTFPT SECTSDVEPD TREMVRAQNK KKKKSGGFQS MGLSYPVFKG IMKKGYKVPT121PIQRKTIPVI LDGKDVVAMA RTGSGKTACF LLPMFERLKT HSAQTGARAL ILSPTRELAL181QTLKFTKELG KFTGLKTALI LGGDRMEDQF AALHENPDII IATPGRLVHV AVEMSLKLQS241VEYVVFDEAD RLFEMGFAEQ LQEIIARLPG GHQTVLFSAT LPKLLVEFAR AGLTEPVLIR301LDVDTKLNEQ LKTSFFLVRE DTKAAVLLHL LHNVVRPQDQ TVVFVATKHH AEYLTELLTT361QRVSCAHIYS ALDPTARKIN LAKFTLGKCS TLIVTDLAAR GLDIPLLDNV INYSFPAKGK421LFLHRVGRVA RAGRSGTAYS LVAPDEIPYL LDLHLFLGRS LTLARPLKEP SGVAGVDGML481GRVPQSVVDE EDSGLQSTLE ASLELRGLAR VADNAQQQYV RSRPAPSPES IKRAKEMDLV541GLGLHPLFSS RFEEEELQRL RLVDSIKNYR SRATIFEINA SSRDLCSQVM RAKRQKDRKA601IARFQQGQQG RQEQQEGPVG PAPSRPALQE KQPEKEEEEE AGESVEDIFS EVVGRKRQRS661GPNRGAKRRR EEARQRDQEF YIPYRPKDFD SERGLSISGE GGAFEQQAAG AVLDLMGDEA721QNLTRGRQQL KWDRKKKRFV GQSGQEDKKK IKTESGRYIS SSYKRDLYQK WKQKQKIDDR781DSDEEGASDR RGPERRGGKR DRGQAGASRP HAPGTPAGRV RPELKTKQQI LKQRRRAQKL841HFLQRGGLKQ LSARNRRRVQ ELQQGAFGRG ARSKKGKMRK RMTRTRPLEQKLISEEDLAANDILDYKDDDDKV
882 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.