Human DOPEY2 ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F50802-1
1 plate / $915.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DOPEY2 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G89339-20
Catalog Number: LS-G89339
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DOPEY2 (NM_005128) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- 21orf5 | DOPEY2 | Homolog of yeast DOP1 | KIAA0933 | Protein dopey-2 | C21orf5 | Dopey family member 2
- Origin Species
- Human
- NCBI GenBank
- NM_005128
- SwissProt
- Q9Y3R5
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 258.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDPEEQELLN DYRYRSYSSV IEKALRNFES SSEWADLISS LGKLNKALQS NLRYSLLPRR61LLISKRLAQC LHPALPSGVH LKALETYEII FKIVGTKWLA KDLFLYSCGL FPLLAHAAVS121VRPVLLTLYE KYFLPLQKLL LPSLQAFIVG LLPGLEEGSE ISDRTDALLL RLSLVVGKEV181FYTALWGSVL ASPSIRLPAS VFVVGHINRD APGREQKYML GTNHQLTVKS LRASLLDSNV241LVQRNNLEIV LFFFPFYTCL DSNERAIPLL RSDIVRILSA ATQTLLRRDM SLNRRLYAWL301LGSDIKGNTV VPESEISNSY EDQSSYFFEK YSKDLLVEGL AEILHQKFID ADVEERHHAY361LKPFRVLISL LDKPEIGPQV VGNLFLEVIR AFYSYCRDAL GSDLKLSYTQ SGNSLISAIK421ENRNASEIVK TVNLLITSLS TDFLWDYMTR CFEECFRPVK QRYSVRNSVS PPPTVSELCA481LLVFLLDVIP LELYSEVQTQ YLPQVLGCLV QPLAEDMEAL SLPELTHALK TCFKVLSKVQ541MPPSYLDTES TSGTSSPVKG ENGKIILETK AVIPGDEDAS FPPLKSEDSG IGLSASSPEL601SEHLRVPRVS LERDDVWKKG GSMQRTFLCI QELIANFASK NIFGVQLTAS GEESKSEEPA661GKRDRDGTQS LAANDSSRKN SWEPKPITVP QFKQMLSDLF TARGSPFKTK SSESPSSSPS721SPARKNGGEW DVEKVVIDLG GSREERREAF AAACHLLLDC ATFPVYLSEE ETEQLCATLF781QLPGAGDSSF PSWLKSLMTI CCCVTDCYLQ NVAISTLLEV INHSQSLALV IEDKMKRYKS841SGHNPFFGKL QMVTVPPIAP GILKVIAEKT DFYQRVARVL WNQLNKETRE HHVTCVELFY901RLHCLAPTAN ICEDIICHAL LDPDKGTRLE ALFRFSVIWH LTREIQGSRV TSHNRSFDRS961LFVVLDSLAC TDGAIGAAAQ GWLVRALSLG DVARILEPVL LLLLQPKTQR TSIHCLKQEN1021SADDLHRWFN RKKTSFREAC AVPEPQESGS EEHLPLSQFT TVDREAIWAE VEKEPEKYPL1081RGELSEEELP YYVELPDRTA HGAPDSSEHT ESADTSSCHT DSENTSSFSS PSHDLQELSN1141EENCCAPIPM GGRAYPKRSA LLAAFQSESF KAGAKLSLVR VDSDKTQASE SFSSDEEADL1201ELQALTTSRL LKQQRERQEA VEALFKHILL YLQPYDSRRV LYAFSVLEAV LKTNPKEFIE1261AVSRTSMDTS STAHLNLISN LLARHQEALI GQSFYGKLQT QVPNVCPHSL LLELLTYLCL1321SFLRSYYPCY LKVSHRDILG NRDVQVKSVE VLIRIMMQLV SVAKSSEGKN VEFIHSLLQR1381CKVQEFVLLS LSASMYTSQK RYGLATAHHG RALPEDSLFE ESLINLGQDQ IWSEHPLQIE1441LLKLLQVLIV LEHHLGRAHE EAENQPDLSR EWQRALNFQQ AISALQYVQP HPLTSQGLLV1501SAVVRGLQPA YGYGMHPAWV SLVTHSLPYF GKSLGWTVTP FVVQICKNLD DLVKQYESES1561VKLSVSTTSK RENISPDYPL TLLEGLTTIS HFCLLEQANQ NKKTMAAGDP ANLRNARNAI1621LEELPRTVNT MALLWNVLRK EETQKRPVDL LGATKGSSSV YFKTTKTIRQ KILDFLNPLT1681AHLGVQLTAA VAAVWSRKKA QRHSKMKIIP TASASQLTLV DLVCALSTLQ TDTLLHLVKE1741VVKRPPQVKG GDEKSPLVDI PVLQFCYAFL QRLPVPALQE NFSSLLGVLK ESVQLNLAPP1801GYFLLLSMLN DFVTRTPNLE NKKDQKDLQE ITQKILEAVG NIAGSSLEQT SWLSRNLEVK1861AQPQASLEES DAEEDLYDAA AASAMVSSSA PSVYSVQALS LLAEVLASLL DMVYRSDEKE1921KAVPLISRLL YYVFPYLRNH SAYNAPSFRA GAQLLSSLSG YAYTKRAWRK EVLELFLDPA1981FFQMDTSCVH WKSIIDHLLT HEKTMFKDLM NMQSSSLKLF SSFEQKAMLL KRQAFAVFSG2041ELDQYHLYLP LIQERLTDNL RVGQTSIVAA QMFLFFRVLL LRISPQHLTS LWPIMVSELI2101QTFTQLEEDL KDEDESLRST NKVNRTKVSV PDANGPSVGE IPQSELILYL SACKFLDTAL2161SFPPDKMPLF QIYRWAFIPE VDTEGPAFLS DVEENHQECK PHTVRILELL KLKFGEISSS2221DEITMKSEFP LLRQHSVSSI RQLMPFFMTL NGAFKTQRQL PADSPGTPFL DFPVTDSPRI2281LKQLEECIEY DFLEHPECTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2298 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
