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DOPEY2 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DOPEY2 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DOPEY2 antibodies →
$725.00each
Catalog Number: LS-G116146-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DOPEY2 (NM_005128), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
21orf5 | DOPEY2 | Homolog of yeast DOP1 | KIAA0933 | Protein dopey-2 | C21orf5 | Dopey family member 2
Origin Species
Human
NCBI GenBank
NM_005128
SwissProt
Q9Y3R5

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
258.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDPEEQELLN DYRYRSYSSV IEKALRNFES SSEWADLISS LGKLNKALQS NLRYSLLPRR
61LLISKRLAQC LHPALPSGVH LKALETYEII FKIVGTKWLA KDLFLYSCGL FPLLAHAAVS
121VRPVLLTLYE KYFLPLQKLL LPSLQAFIVG LLPGLEEGSE ISDRTDALLL RLSLVVGKEV
181FYTALWGSVL ASPSIRLPAS VFVVGHINRD APGREQKYML GTNHQLTVKS LRASLLDSNV
241LVQRNNLEIV LFFFPFYTCL DSNERAIPLL RSDIVRILSA ATQTLLRRDM SLNRRLYAWL
301LGSDIKGNTV VPESEISNSY EDQSSYFFEK YSKDLLVEGL AEILHQKFID ADVEERHHAY
361LKPFRVLISL LDKPEIGPQV VGNLFLEVIR AFYSYCRDAL GSDLKLSYTQ SGNSLISAIK
421ENRNASEIVK TVNLLITSLS TDFLWDYMTR CFEECFRPVK QRYSVRNSVS PPPTVSELCA
481LLVFLLDVIP LELYSEVQTQ YLPQVLGCLV QPLAEDMEAL SLPELTHALK TCFKVLSKVQ
541MPPSYLDTES TSGTSSPVKG ENGKIILETK AVIPGDEDAS FPPLKSEDSG IGLSASSPEL
601SEHLRVPRVS LERDDVWKKG GSMQRTFLCI QELIANFASK NIFGVQLTAS GEESKSEEPA
661GKRDRDGTQS LAANDSSRKN SWEPKPITVP QFKQMLSDLF TARGSPFKTK SSESPSSSPS
721SPARKNGGEW DVEKVVIDLG GSREERREAF AAACHLLLDC ATFPVYLSEE ETEQLCATLF
781QLPGAGDSSF PSWLKSLMTI CCCVTDCYLQ NVAISTLLEV INHSQSLALV IEDKMKRYKS
841SGHNPFFGKL QMVTVPPIAP GILKVIAEKT DFYQRVARVL WNQLNKETRE HHVTCVELFY
901RLHCLAPTAN ICEDIICHAL LDPDKGTRLE ALFRFSVIWH LTREIQGSRV TSHNRSFDRS
961LFVVLDSLAC TDGAIGAAAQ GWLVRALSLG DVARILEPVL LLLLQPKTQR TSIHCLKQEN
1021SADDLHRWFN RKKTSFREAC AVPEPQESGS EEHLPLSQFT TVDREAIWAE VEKEPEKYPL
1081RGELSEEELP YYVELPDRTA HGAPDSSEHT ESADTSSCHT DSENTSSFSS PSHDLQELSN
1141EENCCAPIPM GGRAYPKRSA LLAAFQSESF KAGAKLSLVR VDSDKTQASE SFSSDEEADL
1201ELQALTTSRL LKQQRERQEA VEALFKHILL YLQPYDSRRV LYAFSVLEAV LKTNPKEFIE
1261AVSRTSMDTS STAHLNLISN LLARHQEALI GQSFYGKLQT QVPNVCPHSL LLELLTYLCL
1321SFLRSYYPCY LKVSHRDILG NRDVQVKSVE VLIRIMMQLV SVAKSSEGKN VEFIHSLLQR
1381CKVQEFVLLS LSASMYTSQK RYGLATAHHG RALPEDSLFE ESLINLGQDQ IWSEHPLQIE
1441LLKLLQVLIV LEHHLGRAHE EAENQPDLSR EWQRALNFQQ AISALQYVQP HPLTSQGLLV
1501SAVVRGLQPA YGYGMHPAWV SLVTHSLPYF GKSLGWTVTP FVVQICKNLD DLVKQYESES
1561VKLSVSTTSK RENISPDYPL TLLEGLTTIS HFCLLEQANQ NKKTMAAGDP ANLRNARNAI
1621LEELPRTVNT MALLWNVLRK EETQKRPVDL LGATKGSSSV YFKTTKTIRQ KILDFLNPLT
1681AHLGVQLTAA VAAVWSRKKA QRHSKMKIIP TASASQLTLV DLVCALSTLQ TDTLLHLVKE
1741VVKRPPQVKG GDEKSPLVDI PVLQFCYAFL QRLPVPALQE NFSSLLGVLK ESVQLNLAPP
1801GYFLLLSMLN DFVTRTPNLE NKKDQKDLQE ITQKILEAVG NIAGSSLEQT SWLSRNLEVK
1861AQPQASLEES DAEEDLYDAA AASAMVSSSA PSVYSVQALS LLAEVLASLL DMVYRSDEKE
1921KAVPLISRLL YYVFPYLRNH SAYNAPSFRA GAQLLSSLSG YAYTKRAWRK EVLELFLDPA
1981FFQMDTSCVH WKSIIDHLLT HEKTMFKDLM NMQSSSLKLF SSFEQKAMLL KRQAFAVFSG
2041ELDQYHLYLP LIQERLTDNL RVGQTSIVAA QMFLFFRVLL LRISPQHLTS LWPIMVSELI
2101QTFTQLEEDL KDEDESLRST NKVNRTKVSV PDANGPSVGE IPQSELILYL SACKFLDTAL
2161SFPPDKMPLF QIYRWAFIPE VDTEGPAFLS DVEENHQECK PHTVRILELL KLKFGEISSS
2221DEITMKSEFP LLRQHSVSSI RQLMPFFMTL NGAFKTQRQL PADSPGTPFL DFPVTDSPRI
2281LKQLEECIEY DFLEHPEC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2298 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.