DOCK6 Antibody
Catalog Number: LS-C665172-100
100 µg (2 mg/mL) / $359.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DOCK6 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95627-20
Catalog Number: LS-G95627
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Dedicator of cytokinesis protein 6(DOCK6) (NM_020812) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- SwissProt
- Q96HP0
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 229.6 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAASERRAFA HKINRTVAAE VRKQVSRERS GSPHSSRRCS SSLGVPLTEV VEPLDFEDVL61LSRPPDAEPG PLRDLVEFPA DDLELLLQPR ECRTTEPGIP KDEKLDAQVR AAVEMYIEDW121VIVHRRYQYL SAAYSPVTTD TQRERQKGLP RQVFEQDASG DERSGPEDSN DSRRGSGSPE181DTPRSSGASS IFDLRNLAAD SLLPSLLERA APEDVDRRNE TLRRQHRPPA LLTLYPAPDE241DEAVERCSRP EPPREHFGQR ILVKCLSLKF EIEIEPIFGI LALYDVREKK KISENFYFDL301NSDSMKGLLR AHGTHPAIST LARSAIFSVT YPSPDIFLVI KLEKVLQQGD ISECCEPYMV361LKEVDTAKNK EKLEKLRLAA EQFCTRLGRY RMPFAWTAVH LANIVSSAGQ LDRDSDSEGE421RRPAWTDRRR RGPQDRASSG DDACSFSGFR PATLTVTNFF KQEAERLSDE DLFKFLADMR481RPSSLLRRLR PVTAQLKIDI SPAPENPHFC LSPELLHIKP YPDPRGRPTK EILEFPAREV541YAPHTSYRNL LYVYPHSLNF SSRQGSVRNL AVRVQYMTGE DPSQALPVIF GKSSCSEFTR601EAFTPVVYHN KSPEFYEEFK LHLPACVTEN HHLLFTFYHV SCQPRPGTAL ETPVGFTWIP661LLQHGRLRTG PFCLPVSVDQ PPPSYSVLTP DVALPGMRWV DGHKGVFSVE LTAVSSVHPQ721DPYLDKFFTL VHVLEEGAFP FRLKDTVLSE GNVEQELRAS LAALRLASPE PLVAFSHHVL781DKLVRLVIRP PIISGQIVNL GRGAFEAMAH VVSLVHRSLE AAQDARGHCP QLAAYVHYAF841RLPGTEPSLP DGAPPVTVQA ATLARGSGRP ASLYLARSKS ISSSNPDLAV APGSVDDEVS901RILASKLLHE ELALQWVVSS SAVREAILQH AWFFFQLMVK SMALHLLLGQ RLDTPRKLRF961PGRFLDDITA LVGSVGLEVI TRVHKDVELA EHLNASLAFF LSDLLSLVDR GFVFSLVRAH1021YKQVATRLQS SPNPAALLTL RMEFTRILCS HEHYVTLNLP CCPLSPPASP SPSVSSTTSQ1081SSTFSSQAPD PKVTSMFELS GPFRQQHFLA GLLLTELALA LEPEAEGAFL LHKKAISAVH1141SLLCGHDTDP RYAEATVKAR VAELYLPLLS IARDTLPRLH DFAEGPGQRS RLASMLDSDT1201EGEGDIAGTI NPSVAMAIAG GPLAPGSRAS ISQGPPTASR AGCALSAESS RTLLACVLWV1261LKNTEPALLQ RWATDLTLPQ LGRLLDLLYL CLAAFEYKGK KAFERINSLT FKKSLDMKAR1321LEEAILGTIG ARQEMVRRSR ERSPFGNPEN VRWRKSVTHW KQTSDRVDKT KDEMEHEALV1381EGNLATEASL VVLDTLEIIV QTVMLSEARE SVLGAVLKVV LYSLGSAQSA LFLQHGLATQ1441RALVSKFPEL LFEEDTELCA DLCLRLLRHC GSRISTIRTH ASASLYLLMR QNFEIGHNFA1501RVKMQVTMSL SSLVGTTQNF SEEHLRRSLK TILTYAEEDM GLRDSTFAEQ VQDLMFNLHM1561ILTDTVKMKE HQEDPEMLID LMYRIARGYQ GSPDLRLTWL QNMAGKHAEL GNHAEAAQCM1621VHAAALVAEY LALLEDHRHL PVGCVSFQNI SSNVLEESAI SDDILSPDEE GFCSGKHFTE1681LGLVGLLEQA AGYFTMGGLY EAVNEVYKNL IPILEAHRDY KKLAAVHGKL QEAFTKIMHQ1741SSGWERVFGT YFRVGFYGAH FGDLDEQEFV YKEPSITKLA EISHRLEEFY TERFGDDVVE1801IIKDSNPVDK SKLDSQKAYI QITYVEPYFD TYELKDRVTY FDRNYGLRTF LFCTPFTPDG1861RAHGELPEQH KRKTLLSTDH AFPYIKTRIR VCHREETVLT PVEVAIEDMQ KKTRELAFAT1921EQDPPDAKML QMVLQGSVGP TVNQGPLEVA QVFLAEIPED PKLFRHHNKL RLCFKDFCKK1981CEDALRKNKA LIGPDQKEYH RELERNYCRL REALQPLLTQ RLPQLMAPTP PGLRNSLNRA2041SFRKADLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
2047 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
