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DOCK6 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DOCK6 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DOCK6 antibodies →
$725.00each
Catalog Number: LS-G110968-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dedicator of cytokinesis protein 6(DOCK6) (NM_020812), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
SwissProt
Q96HP0

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
229.6 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASERRAFA HKINRTVAAE VRKQVSRERS GSPHSSRRCS SSLGVPLTEV VEPLDFEDVL
61LSRPPDAEPG PLRDLVEFPA DDLELLLQPR ECRTTEPGIP KDEKLDAQVR AAVEMYIEDW
121VIVHRRYQYL SAAYSPVTTD TQRERQKGLP RQVFEQDASG DERSGPEDSN DSRRGSGSPE
181DTPRSSGASS IFDLRNLAAD SLLPSLLERA APEDVDRRNE TLRRQHRPPA LLTLYPAPDE
241DEAVERCSRP EPPREHFGQR ILVKCLSLKF EIEIEPIFGI LALYDVREKK KISENFYFDL
301NSDSMKGLLR AHGTHPAIST LARSAIFSVT YPSPDIFLVI KLEKVLQQGD ISECCEPYMV
361LKEVDTAKNK EKLEKLRLAA EQFCTRLGRY RMPFAWTAVH LANIVSSAGQ LDRDSDSEGE
421RRPAWTDRRR RGPQDRASSG DDACSFSGFR PATLTVTNFF KQEAERLSDE DLFKFLADMR
481RPSSLLRRLR PVTAQLKIDI SPAPENPHFC LSPELLHIKP YPDPRGRPTK EILEFPAREV
541YAPHTSYRNL LYVYPHSLNF SSRQGSVRNL AVRVQYMTGE DPSQALPVIF GKSSCSEFTR
601EAFTPVVYHN KSPEFYEEFK LHLPACVTEN HHLLFTFYHV SCQPRPGTAL ETPVGFTWIP
661LLQHGRLRTG PFCLPVSVDQ PPPSYSVLTP DVALPGMRWV DGHKGVFSVE LTAVSSVHPQ
721DPYLDKFFTL VHVLEEGAFP FRLKDTVLSE GNVEQELRAS LAALRLASPE PLVAFSHHVL
781DKLVRLVIRP PIISGQIVNL GRGAFEAMAH VVSLVHRSLE AAQDARGHCP QLAAYVHYAF
841RLPGTEPSLP DGAPPVTVQA ATLARGSGRP ASLYLARSKS ISSSNPDLAV APGSVDDEVS
901RILASKLLHE ELALQWVVSS SAVREAILQH AWFFFQLMVK SMALHLLLGQ RLDTPRKLRF
961PGRFLDDITA LVGSVGLEVI TRVHKDVELA EHLNASLAFF LSDLLSLVDR GFVFSLVRAH
1021YKQVATRLQS SPNPAALLTL RMEFTRILCS HEHYVTLNLP CCPLSPPASP SPSVSSTTSQ
1081SSTFSSQAPD PKVTSMFELS GPFRQQHFLA GLLLTELALA LEPEAEGAFL LHKKAISAVH
1141SLLCGHDTDP RYAEATVKAR VAELYLPLLS IARDTLPRLH DFAEGPGQRS RLASMLDSDT
1201EGEGDIAGTI NPSVAMAIAG GPLAPGSRAS ISQGPPTASR AGCALSAESS RTLLACVLWV
1261LKNTEPALLQ RWATDLTLPQ LGRLLDLLYL CLAAFEYKGK KAFERINSLT FKKSLDMKAR
1321LEEAILGTIG ARQEMVRRSR ERSPFGNPEN VRWRKSVTHW KQTSDRVDKT KDEMEHEALV
1381EGNLATEASL VVLDTLEIIV QTVMLSEARE SVLGAVLKVV LYSLGSAQSA LFLQHGLATQ
1441RALVSKFPEL LFEEDTELCA DLCLRLLRHC GSRISTIRTH ASASLYLLMR QNFEIGHNFA
1501RVKMQVTMSL SSLVGTTQNF SEEHLRRSLK TILTYAEEDM GLRDSTFAEQ VQDLMFNLHM
1561ILTDTVKMKE HQEDPEMLID LMYRIARGYQ GSPDLRLTWL QNMAGKHAEL GNHAEAAQCM
1621VHAAALVAEY LALLEDHRHL PVGCVSFQNI SSNVLEESAI SDDILSPDEE GFCSGKHFTE
1681LGLVGLLEQA AGYFTMGGLY EAVNEVYKNL IPILEAHRDY KKLAAVHGKL QEAFTKIMHQ
1741SSGWERVFGT YFRVGFYGAH FGDLDEQEFV YKEPSITKLA EISHRLEEFY TERFGDDVVE
1801IIKDSNPVDK SKLDSQKAYI QITYVEPYFD TYELKDRVTY FDRNYGLRTF LFCTPFTPDG
1861RAHGELPEQH KRKTLLSTDH AFPYIKTRIR VCHREETVLT PVEVAIEDMQ KKTRELAFAT
1921EQDPPDAKML QMVLQGSVGP TVNQGPLEVA QVFLAEIPED PKLFRHHNKL RLCFKDFCKK
1981CEDALRKNKA LIGPDQKEYH RELERNYCRL REALQPLLTQ RLPQLMAPTP PGLRNSLNRA
2041SFRKADL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

2047 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.