LSBio
Human DNM3 / Dynamin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DNM3 / Dynamin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DNM3 antibodies →
$220.00each
Catalog Number: LS-G83584-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Dynamin 3(DNM3) (NM_015569) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DNM3 | Dyna III | Dynamin 3 | Dynamin family member | Dynamin-3 | Dynamin | testicular | KIAA0820 | T-dynamin
Origin Species
Human
NCBI GenBank
AL136712
SwissProt
Q9UQ16

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
96.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MGNREMEELI PLVNRLQDAF SALGQSCLLE LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG
61SGIVTRRPLV LQLVTSKAEY AEFLHCKGKK FTDFDEVRLE IEAETDRVTG MNKGISSIPI
121NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIREMI MQFITRENCL ILAVTPANTD
181LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YVGVVNRSQK
241DIDGKKDIKA AMLAERKFFL SHPAYRHIAD RMGTPHLQKV LNQQLTNHIR DTLPNFRNKL
301QGQLLSIEHE VEAYKNFKPE DPTRKTKALL QMVQQFAVDF EKRIEGSGDQ VDTLELSGGA
361KINRIFHERF PFEIVKMEFN EKELRREISY AIKNIHGIRT GLFTPDMAFE AIVKKQIVKL
421KGPSLKSVDL VIQELINTVK KCTKKLANFP RLCEETERIV ANHIREREGK TKDQVLLLID
481IQVSYINTNH EDFIGFANAQ QRSSQVHKKT TVGNQVIRKG WLTISNIGIM KGGSKGYWFV
541LTAESLSWYK DDEEKEKKYM LPLDNLKVRD VEKSFMSSKH IFALFNTEQR NVYKDYRFLE
601LACDSQEDVD SWKASLLRAG VYPDKSVGNN KAENDENGQA ENFSMDPQLE RQVETIRNLV
661DSYMSIINKC IRDLIPKTIM HLMINNVKDF INSELLAQLY SSEDQNTLME ESAEQAQRRD
721EMLRMYQALK EALGIIGDIS TATVSTPAPP PVDDSWIQHS RRSPPPSPTT QRRPTLSAPL
781ARPTSGRGPA PAIPSPGPHS GAPPVPFRPG PLPPFPSSSD SFGAPPQVPS RPTRAPPSVP
841SRRPPPSPTR PTIIRPLESS LLDSGP
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

866 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.