Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DNM3 / Dynamin 3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G101334-100
Catalog Number: LS-G101334
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Dynamin 3(DNM3) (NM_015569), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DNM3 | Dyna III | Dynamin 3 | Dynamin family member | Dynamin-3 | Dynamin | testicular | KIAA0820 | T-dynamin
- Origin Species
- Human
- NCBI GenBank
- AL136712
- SwissProt
- Q9UQ16
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 96.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MGNREMEELI PLVNRLQDAF SALGQSCLLE LPQIAVVGGQ SAGKSSVLEN FVGRDFLPRG61SGIVTRRPLV LQLVTSKAEY AEFLHCKGKK FTDFDEVRLE IEAETDRVTG MNKGISSIPI121NLRVYSPHVL NLTLIDLPGI TKVPVGDQPP DIEYQIREMI MQFITRENCL ILAVTPANTD181LANSDALKLA KEVDPQGLRT IGVITKLDLM DEGTDARDVL ENKLLPLRRG YVGVVNRSQK241DIDGKKDIKA AMLAERKFFL SHPAYRHIAD RMGTPHLQKV LNQQLTNHIR DTLPNFRNKL301QGQLLSIEHE VEAYKNFKPE DPTRKTKALL QMVQQFAVDF EKRIEGSGDQ VDTLELSGGA361KINRIFHERF PFEIVKMEFN EKELRREISY AIKNIHGIRT GLFTPDMAFE AIVKKQIVKL421KGPSLKSVDL VIQELINTVK KCTKKLANFP RLCEETERIV ANHIREREGK TKDQVLLLID481IQVSYINTNH EDFIGFANAQ QRSSQVHKKT TVGNQVIRKG WLTISNIGIM KGGSKGYWFV541LTAESLSWYK DDEEKEKKYM LPLDNLKVRD VEKSFMSSKH IFALFNTEQR NVYKDYRFLE601LACDSQEDVD SWKASLLRAG VYPDKSVGNN KAENDENGQA ENFSMDPQLE RQVETIRNLV661DSYMSIINKC IRDLIPKTIM HLMINNVKDF INSELLAQLY SSEDQNTLME ESAEQAQRRD721EMLRMYQALK EALGIIGDIS TATVSTPAPP PVDDSWIQHS RRSPPPSPTT QRRPTLSAPL781ARPTSGRGPA PAIPSPGPHS GAPPVPFRPG PLPPFPSSSD SFGAPPQVPS RPTRAPPSVP841SRRPPPSPTR PTIIRPLESS LLDSGPSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
866 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.