LSBio
Human DNER / BET Protein (Over-Expression Lysate Myc-DDK (Flag))

Human DNER / BET Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DNER antibodies →
$153.00each
Catalog Number: LS-G82841-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DNER (NM_139072) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Bet | DNER | H_NH0150O02.1 | UNQ26 | WUGSC:H_NH0150O02.1
Origin Species
Human
NCBI GenBank
E15003
SwissProt
Q8NFT8

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
78.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MQPRRAQAPG AQLLPALALL LLLLGAGPRG SSLANPVPAA PLSAPGPCAA QPCRNGGVCT
61SRPEPDPQHP APAGEPGYSC TCPAGISGAN CQLVADPCAS NPCHHGNCSS SSSSSSDGYL
121CICNEGYEGP NCEQALPSLP ATGWTESMAP RQLQPVPATQ EPDKILPRSQ ATVTLPTWQP
181KTGQKVVEMK WDQVEVIPDI ACGNASSNSS AGGRLVSFEV PQNTSVKIRQ DATASLILLW
241KVTATGFQQC SLIDGRSVTP LQASGGLVLL EEMLALGNNH FIGFVNDSVT KSIVALRLTL
301VVKVSTCVPG ESHANDLECS GKGKCTTKPS EATFSCTCEE QYVGTFCEEY DACQRKPCQN
361NASCIDANEK QDGSNFTCVC LPGYTGELCQ SKIDYCILDP CRNGATCISS LSGFTCQCPE
421GYFGSACEEK VDPCASSPCQ NNGTCYVDGV HFTCNCSPGF TGPTCAQLID FCALSPCAHG
481TCRSVGTSYK CLCDPGYHGL YCEEEYNECL SAPCLNAATC RDLVNGYECV CLAEYKGTHC
541ELYKDPCANV SCLNGATCDS DGLNGTCICA PGFTGEECDI DINECDSNPC HHGGSCLDQP
601NGYNCHCPHG WVGANCEIHL QWKSGHMAES LTNMPRHSLY IIIGALCVAF ILMLIILIVG
661ICRISRIEYQ GSSRPAYEEF YNCRSIDSEF SNAIASIRHA RFGKKSRPAM YDVSPIAYED
721YSPDDKPLVT LIKTKDL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

737 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.