DNER / BET Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G107125-100
Catalog Number: LS-G107125
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DNER (NM_139072), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Bet | DNER | H_NH0150O02.1 | UNQ26 | WUGSC:H_NH0150O02.1
- Origin Species
- Human
- NCBI GenBank
- E15003
- SwissProt
- Q8NFT8
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 78.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MQPRRAQAPG AQLLPALALL LLLLGAGPRG SSLANPVPAA PLSAPGPCAA QPCRNGGVCT61SRPEPDPQHP APAGEPGYSC TCPAGISGAN CQLVADPCAS NPCHHGNCSS SSSSSSDGYL121CICNEGYEGP NCEQALPSLP ATGWTESMAP RQLQPVPATQ EPDKILPRSQ ATVTLPTWQP181KTGQKVVEMK WDQVEVIPDI ACGNASSNSS AGGRLVSFEV PQNTSVKIRQ DATASLILLW241KVTATGFQQC SLIDGRSVTP LQASGGLVLL EEMLALGNNH FIGFVNDSVT KSIVALRLTL301VVKVSTCVPG ESHANDLECS GKGKCTTKPS EATFSCTCEE QYVGTFCEEY DACQRKPCQN361NASCIDANEK QDGSNFTCVC LPGYTGELCQ SKIDYCILDP CRNGATCISS LSGFTCQCPE421GYFGSACEEK VDPCASSPCQ NNGTCYVDGV HFTCNCSPGF TGPTCAQLID FCALSPCAHG481TCRSVGTSYK CLCDPGYHGL YCEEEYNECL SAPCLNAATC RDLVNGYECV CLAEYKGTHC541ELYKDPCANV SCLNGATCDS DGLNGTCICA PGFTGEECDI DINECDSNPC HHGGSCLDQP601NGYNCHCPHG WVGANCEIHL QWKSGHMAES LTNMPRHSLY IIIGALCVAF ILMLIILIVG661ICRISRIEYQ GSSRPAYEEF YNCRSIDSEF SNAIASIRHA RFGKKSRPAM YDVSPIAYED721YSPDDKPLVT LIKTKDLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
737 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.