DNAAF3 Antibody (Biotin)
Catalog Number: LS-C677686
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DNAAF3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G96246-20
Catalog Number: LS-G96246
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human C19orf51(DNAAF3) (NM_178837) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Origin Species
- Human
- NCBI GenBank
- NM_001256714
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MLPLLDSSKR AGTLGSGCGV PRVHSAALSR EEGASRDIWR IKVWARVMTT PAGSGSGFGS61VSWWGLSPAL DLQAESPPVD PDSQADTVHS NPELDVLLLG SVDGRHLLRT LSRAKFWPRR121RFNFFVLENN LEAVARHMLI FSLALEEPEK MGLQERSETF LEVWGNALLR PPVAAFVRAQ181ADLLAHLVPE PDRLEEQLPW LSLRALKFRE RDALEAVFRF WAGGEKGPQA FPMSRLWDSR241LRHYLGSRYD ARRGVSDWDL RMKLHDRGAQ VIHPQEFRRW RDTGVAFELR DSSAYHVPNR301TLASGRLLSY RGERVAARGY WGDIATGPFV AFGIEADDES LLRTSNGQPV KTAGEITQHN361VTELLRDVAA WGRARATGGD LEEQQHAEGS PEPGTPAAPT PESFTVHFLP LNSAQTLHHK421SCYNGRFQLL YVACGMVHLL IPELGACVAP GGNLIVELAR YLVDVRQEQL QGFNTRVREL481AQAAGFAPQT GARPSETFAR FCKSQESALG NTVPAVEPGT PPLDILAQPL EASNPALEGL541TQPLQGGTPH CEPCQLPSES PGSLSEVLAQ PQGALAPPNC ESDSKTGVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
588 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
