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DNAAF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DNAAF3 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$725.00each
Catalog Number: LS-G104602-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human C19orf51(DNAAF3) (NM_178837), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Origin Species
Human
NCBI GenBank
NM_001256714

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MLPLLDSSKR AGTLGSGCGV PRVHSAALSR EEGASRDIWR IKVWARVMTT PAGSGSGFGS
61VSWWGLSPAL DLQAESPPVD PDSQADTVHS NPELDVLLLG SVDGRHLLRT LSRAKFWPRR
121RFNFFVLENN LEAVARHMLI FSLALEEPEK MGLQERSETF LEVWGNALLR PPVAAFVRAQ
181ADLLAHLVPE PDRLEEQLPW LSLRALKFRE RDALEAVFRF WAGGEKGPQA FPMSRLWDSR
241LRHYLGSRYD ARRGVSDWDL RMKLHDRGAQ VIHPQEFRRW RDTGVAFELR DSSAYHVPNR
301TLASGRLLSY RGERVAARGY WGDIATGPFV AFGIEADDES LLRTSNGQPV KTAGEITQHN
361VTELLRDVAA WGRARATGGD LEEQQHAEGS PEPGTPAAPT PESFTVHFLP LNSAQTLHHK
421SCYNGRFQLL YVACGMVHLL IPELGACVAP GGNLIVELAR YLVDVRQEQL QGFNTRVREL
481AQAAGFAPQT GARPSETFAR FCKSQESALG NTVPAVEPGT PPLDILAQPL EASNPALEGL
541TQPLQGGTPH CEPCQLPSES PGSLSEVLAQ PQGALAPPNC ESDSKTGV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

588 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.