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Human DLGAP3 / SAPAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DLGAP3 / SAPAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
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$220.00each
Catalog Number: LS-G88454-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human SAPAP3(DLGAP3) (NM_001080418) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
PSD-95/SAP90-binding protein 3 | DAP-3 | DLGAP3 | SAPAP3
Origin Species
Human
NCBI GenBank
AF131778
SwissProt
O95886

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
105.9 kda
Protein Type
Lysate
Amino Acid Sequence
1MRGYHGDRGS HPRPARFADQ QHMDVGPAAR APYLLGSREA FSTEPRFCAP RAGLGHISPE
61GPLSLSEGPS VGPEGGPAGA GVGGGSSTFP RMYPGQGPFD TCEDCVGHPQ GKGAPRLPPT
121LLDQFEKQLP VQQDGFHTLP YQRGPAGAGP GPAPGTGTAP EPRSESPSRI RHLVHSVQKL
181FAKSHSLEAP GKRDYNGPKA EGRGGSGGDS YPGPGSGGPH TSHHHHHHHH HHHHQSRHGK
241RSKSKDRKGD GRHQAKSTGW WSSDDNLDSD SGFLAGGRPP GEPGGPFCLE GPDGSYRDLS
301FKGRSGGSEG RCLACTGMSM SLDGQSVKRS AWHTMMVSQG RDGYPGAGPG KGLLGPETKA
361KARTYHYLQV PQDDWGGYPT GGKDGEIPCR RMRSGSYIKA MGDEESGDSD GSPKTSPKAV
421ARRFTTRRSS SVDQARINCC VPPRIHPRSS IPGYSRSLTT GQLSDELNQQ LEAVCGSVFG
481ELESQAVDAL DLPGCFRMRS HSYLRAIQAG CSQDDDCLPL LATPAAVSGR PGSSFNFRKA
541PPPIPPGSQA PPRISITAQS STDSAHESFT AAEGPARRCS SADGLDGPAM GARTLELAPV
601PPRASPKPPT LIIKTIPGRE ELRSLARQRK WRPSIGVQVE TISDSDTENR SRREFHSIGV
661QVEEDKRRAR FKRSNSVTAG VQADLELEGL AGLATVATED KALQFGRSFQ RHASEPQPGP
721RAPTYSVFRT VHTQGQWAYR EGYPLPYEPP ATDGSPGPAP APTPGPGAGR RDSWIERGSR
781SLPDSGRASP CPRDGEWFIK MLRAEVEKLE HWCQQMEREA EDYELPEEIL EKIRSAVGST
841QLLLSQKVQQ FFRLCQQSMD PTAFPVPTFQ DLAGFWDLLQ LSIEDVTLKF LELQQLKANS
901WKLLEPKEEK KVPPPIPKKP LRGRGVPVKE RSLDSVDRQR QEARKRLLAA KRAASFRHSS
961ATESADSIEI YIPEAQTRL
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

979 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.