Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DLGAP3 / SAPAP3 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G120206-100
Catalog Number: LS-G120206
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human SAPAP3(DLGAP3) (NM_001080418), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- PSD-95/SAP90-binding protein 3 | DAP-3 | DLGAP3 | SAPAP3
- Origin Species
- Human
- NCBI GenBank
- AF131778
- SwissProt
- O95886
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 105.9 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MRGYHGDRGS HPRPARFADQ QHMDVGPAAR APYLLGSREA FSTEPRFCAP RAGLGHISPE61GPLSLSEGPS VGPEGGPAGA GVGGGSSTFP RMYPGQGPFD TCEDCVGHPQ GKGAPRLPPT121LLDQFEKQLP VQQDGFHTLP YQRGPAGAGP GPAPGTGTAP EPRSESPSRI RHLVHSVQKL181FAKSHSLEAP GKRDYNGPKA EGRGGSGGDS YPGPGSGGPH TSHHHHHHHH HHHHQSRHGK241RSKSKDRKGD GRHQAKSTGW WSSDDNLDSD SGFLAGGRPP GEPGGPFCLE GPDGSYRDLS301FKGRSGGSEG RCLACTGMSM SLDGQSVKRS AWHTMMVSQG RDGYPGAGPG KGLLGPETKA361KARTYHYLQV PQDDWGGYPT GGKDGEIPCR RMRSGSYIKA MGDEESGDSD GSPKTSPKAV421ARRFTTRRSS SVDQARINCC VPPRIHPRSS IPGYSRSLTT GQLSDELNQQ LEAVCGSVFG481ELESQAVDAL DLPGCFRMRS HSYLRAIQAG CSQDDDCLPL LATPAAVSGR PGSSFNFRKA541PPPIPPGSQA PPRISITAQS STDSAHESFT AAEGPARRCS SADGLDGPAM GARTLELAPV601PPRASPKPPT LIIKTIPGRE ELRSLARQRK WRPSIGVQVE TISDSDTENR SRREFHSIGV661QVEEDKRRAR FKRSNSVTAG VQADLELEGL AGLATVATED KALQFGRSFQ RHASEPQPGP721RAPTYSVFRT VHTQGQWAYR EGYPLPYEPP ATDGSPGPAP APTPGPGAGR RDSWIERGSR781SLPDSGRASP CPRDGEWFIK MLRAEVEKLE HWCQQMEREA EDYELPEEIL EKIRSAVGST841QLLLSQKVQQ FFRLCQQSMD PTAFPVPTFQ DLAGFWDLLQ LSIEDVTLKF LELQQLKANS901WKLLEPKEEK KVPPPIPKKP LRGRGVPVKE RSLDSVDRQR QEARKRLLAA KRAASFRHSS961ATESADSIEI YIPEAQTRLTRTRPLEQKLISEEDLAANDILDYKDDDDKV
979 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.