DIAPH1 Antibody
Catalog Number: LS-C334279
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DIAPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G80651-20
Catalog Number: LS-G80651
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DIAPH1 (NM_001079812) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DRF1 | DIA1 | Diaphanous-related formin 1 | Diaphanous-related formin-1 | DIAP1 | Protein diaphanous homolog 1 | DFNA1 | DIAPH1 | HDIA1 | LFHL1
- Origin Species
- Human
- NCBI GenBank
- NM_005219
- SwissProt
- O60610
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 140.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MEPPGGSLGP GRGTRDKKKG RSPDELPSAG GDGGKSKKFL ERFTSMRIKK EKEKPNSAHR61NSSASYGDDP TAQSLQDVSD EQVLVLFEQM LLDMNLNEEK QQPLREKDII IKREMVSQYL121YTSKAGMSQK ESSKSAMMYI QELRSGLRDM PLLSCLESLR VSLNNNPVSW VQTFGAEGLA181SLLDILKRLH DEKEETAGSY DSRNKHEIIR CLKAFMNNKF GIKTMLETEE GILLLVRAMD241PAVPNMMIDA AKLLSALCIL PQPEDMNERV LEAMTERAEM DEVERFQPLL DGLKSGTTIA301LKVGCLQLIN ALITPAEELD FRVHIRSELM RLGLHQVLQD LREIENEDMR VQLNVFDEQG361EEDSYDLKGR LDDIRMEMDD FNEVFQILLN TVKDSKAEPH FLSILQHLLL VRNDYEARPQ421YYKLIEECIS QIVLHKNGAD PDFKCRHLQI EIEGLIDQMI DKTKVEKSEA KAAELEKKLD481SELTARHELQ VEMKKMESDF EQKLQDLQGE KDALHSEKQQ IATEKQDLEA EVSQLTGEVA541KLTKELEDAK KEMASLSAAA ITVPPSVPSR APVPPAPPLP GDSGTIIPPP PAPGDSTTPP601PPPPPPPPPP LPGGVCISSP PSLPGGTAIS PPPPLSGDAT IPPPPPLPEG VGIPSPSSLP661GGTAIPPPPP LPGSARIPPP PPPLPGSAGI PPPPPPLPGE AGMPPPPPPL PGGPGIPPPP721PFPGGPGIPP PPPGMGMPPP PPFGFGVPAA PVLPFGLTPK KLYKPEVQLR RPNWSKLVAE781DLSQDCFWTK VKEDRFENNE LFAKLTLTFS AQTKTSKAKK DQEGGEEKKS VQKKKVKELK841VLDSKTAQNL SIFLGSFRMP YQEIKNVILE VNEAVLTESM IQNLIKQMPE PEQLKMLSEL901KDEYDDLAES EQFGVVMGTV PRLRPRLNAI LFKLQFSEQV ENIKPEIVSV TAACEELRKS961ESFSNLLEIT LLVGNYMNAG SRNAGAFGFN ISFLCKLRDT KSTDQKMTLL HFLAELCEND1021YPDVLKFPDE LAHVEKASRV SAENLQKNLD QMKKQISDVE RDVQNFPAAT DEKDKFVEKM1081TSFVKDAQEQ YNKLRMMHSN METLYKELGE YFLFDPKKLS VEEFFMDLHN FRNMFLQAVK1141ENQKRRETEE KMRRAKLAKE KAEKERLEKQ QKREQLIDMN AEGDETGVMD SLLEALQSGA1201AFRRKRGPRQ ANRKAGCAVT SLLASELTKD DAMAAVPAKV SKNSETFPTI LEEAKELVGR1261ASTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1262 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
