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DIAPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DIAPH1 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DIAPH1 antibodies →
$725.00each
Catalog Number: LS-G120118-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DIAPH1 (NM_001079812), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DRF1 | DIA1 | Diaphanous-related formin 1 | Diaphanous-related formin-1 | DIAP1 | Protein diaphanous homolog 1 | DFNA1 | DIAPH1 | HDIA1 | LFHL1
Origin Species
Human
NCBI GenBank
NM_005219
SwissProt
O60610

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
140.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MEPPGGSLGP GRGTRDKKKG RSPDELPSAG GDGGKSKKFL ERFTSMRIKK EKEKPNSAHR
61NSSASYGDDP TAQSLQDVSD EQVLVLFEQM LLDMNLNEEK QQPLREKDII IKREMVSQYL
121YTSKAGMSQK ESSKSAMMYI QELRSGLRDM PLLSCLESLR VSLNNNPVSW VQTFGAEGLA
181SLLDILKRLH DEKEETAGSY DSRNKHEIIR CLKAFMNNKF GIKTMLETEE GILLLVRAMD
241PAVPNMMIDA AKLLSALCIL PQPEDMNERV LEAMTERAEM DEVERFQPLL DGLKSGTTIA
301LKVGCLQLIN ALITPAEELD FRVHIRSELM RLGLHQVLQD LREIENEDMR VQLNVFDEQG
361EEDSYDLKGR LDDIRMEMDD FNEVFQILLN TVKDSKAEPH FLSILQHLLL VRNDYEARPQ
421YYKLIEECIS QIVLHKNGAD PDFKCRHLQI EIEGLIDQMI DKTKVEKSEA KAAELEKKLD
481SELTARHELQ VEMKKMESDF EQKLQDLQGE KDALHSEKQQ IATEKQDLEA EVSQLTGEVA
541KLTKELEDAK KEMASLSAAA ITVPPSVPSR APVPPAPPLP GDSGTIIPPP PAPGDSTTPP
601PPPPPPPPPP LPGGVCISSP PSLPGGTAIS PPPPLSGDAT IPPPPPLPEG VGIPSPSSLP
661GGTAIPPPPP LPGSARIPPP PPPLPGSAGI PPPPPPLPGE AGMPPPPPPL PGGPGIPPPP
721PFPGGPGIPP PPPGMGMPPP PPFGFGVPAA PVLPFGLTPK KLYKPEVQLR RPNWSKLVAE
781DLSQDCFWTK VKEDRFENNE LFAKLTLTFS AQTKTSKAKK DQEGGEEKKS VQKKKVKELK
841VLDSKTAQNL SIFLGSFRMP YQEIKNVILE VNEAVLTESM IQNLIKQMPE PEQLKMLSEL
901KDEYDDLAES EQFGVVMGTV PRLRPRLNAI LFKLQFSEQV ENIKPEIVSV TAACEELRKS
961ESFSNLLEIT LLVGNYMNAG SRNAGAFGFN ISFLCKLRDT KSTDQKMTLL HFLAELCEND
1021YPDVLKFPDE LAHVEKASRV SAENLQKNLD QMKKQISDVE RDVQNFPAAT DEKDKFVEKM
1081TSFVKDAQEQ YNKLRMMHSN METLYKELGE YFLFDPKKLS VEEFFMDLHN FRNMFLQAVK
1141ENQKRRETEE KMRRAKLAKE KAEKERLEKQ QKREQLIDMN AEGDETGVMD SLLEALQSGA
1201AFRRKRGPRQ ANRKAGCAVT SLLASELTKD DAMAAVPAKV SKNSETFPTI LEEAKELVGR
1261AS
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1262 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.