DHX30 Antibody (Biotin)
Catalog Number: LS-C377871
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general)
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DHX30 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G85802-20
Catalog Number: LS-G85802
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DHX30 (NM_014966) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDX30 | DEAH box protein 30 | KIAA0890 | Retina co-repressor | DHX30 | RETCOR
- Origin Species
- Human
- NCBI GenBank
- NM_014966
- SwissProt
- Q7L2E3
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 129.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAASRDLLKE FPQPKNLLNS VIGRALGISH AKDKLVYVHT NGPKKKKVTL HIKWPKSVEV61EGYGSKKIDA ERQAAAAACQ LFKGWGLLGP RNELFDAAKY RVLADRFGSP ADSWWRPEPT121MPPTSWRQLN PESIRPGGPG GLSRSLGREE EEDEEEELEE GTIDVTDFLS MTQQDSHAPL181RDSRGSSFEM TDDDSAIRAL TQFPLPKNLL AKVIQIATSS STAKNLMQFH TVGTKTKLST241LTLLWPCPMT FVAKGRRKAE AENKAAALAC KKLKSLGLVD RNNEPLTHAM YNLASLRELG301ETQRRPCTIQ VPEPILRKIE TFLNHYPVES SWIAPELRLQ SDDILPLGKD SGPLSDPITG361KPYVPLLEAE EVRLSQSLLE LWRRRGPVWQ EAPQLPVDPH RDTILNAIEQ HPVVVISGDT421GCGKTTRIPQ LLLERYVTEG RGARCNVIIT QPRRISAVSV AQRVSHELGP SLRRNVGFQV481RLESKPPSRG GALLFCTVGI LLRKLQSNPS LEGVSHVIVD EVHERDVNTD FLLILLKGLQ541RLNPALRLVL MSATGDNERF SRYFGGCPVI KVPGFMYPVK EHYLEDILAK LGKHQYLHRH601RHHESEDECA LDLDLVTDLV LHIDARGEPG GILCFLPGWQ EIKGVQQRLQ EALGMHESKY661LILPVHSNIP MMDQKAIFQQ PPVGVRKIVL ATNIAETSIT INDIVHVVDS GLHKEERYDL721KTKVSCLETV WVSRANVIQR RGRAGRCQSG FAYHLFPRSR LEKMVPFQVP EILRTPLENL781VLQAKIHMPE KTAVEFLSKA VDSPNIKAVD EAVILLQEIG VLDQREYLTT LGQRLAHIST841DPRLAKAIVL AAIFRCLHPL LVVVSCLTRD PFSSSLQNRA EVDKVKALLS HDSGSDHLAF901VRAVAGWEEV LRWQDRSSRE NYLEENLLYA PSLRFIHGLI KQFSENIYEA FLVGKPSDCT961LASAQCNEYS EEEELVKGVL MAGLYPNLIQ VRQGKVTRQG KFKPNSVTYR TKSGNILLHK1021STINREATRL RSRWLTYFMA VKSNGSVFVR DSSQVHPLAV LLLTDGDVHI RDDGRRATIS1081LSDSDLLRLE GDSRTVRLLK ELRRALGRMV ERSLRSELAA LPPSVQEEHG QLLALLAELL1141RGPCGSFDVR KTADDTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1155 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
