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DHX30 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DHX30 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DHX30 antibodies →
$504.00each
Catalog Number: LS-G113562-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DHX30 (NM_014966), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX30 | DEAH box protein 30 | KIAA0890 | Retina co-repressor | DHX30 | RETCOR
Origin Species
Human
NCBI GenBank
NM_014966
SwissProt
Q7L2E3

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
129.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAASRDLLKE FPQPKNLLNS VIGRALGISH AKDKLVYVHT NGPKKKKVTL HIKWPKSVEV
61EGYGSKKIDA ERQAAAAACQ LFKGWGLLGP RNELFDAAKY RVLADRFGSP ADSWWRPEPT
121MPPTSWRQLN PESIRPGGPG GLSRSLGREE EEDEEEELEE GTIDVTDFLS MTQQDSHAPL
181RDSRGSSFEM TDDDSAIRAL TQFPLPKNLL AKVIQIATSS STAKNLMQFH TVGTKTKLST
241LTLLWPCPMT FVAKGRRKAE AENKAAALAC KKLKSLGLVD RNNEPLTHAM YNLASLRELG
301ETQRRPCTIQ VPEPILRKIE TFLNHYPVES SWIAPELRLQ SDDILPLGKD SGPLSDPITG
361KPYVPLLEAE EVRLSQSLLE LWRRRGPVWQ EAPQLPVDPH RDTILNAIEQ HPVVVISGDT
421GCGKTTRIPQ LLLERYVTEG RGARCNVIIT QPRRISAVSV AQRVSHELGP SLRRNVGFQV
481RLESKPPSRG GALLFCTVGI LLRKLQSNPS LEGVSHVIVD EVHERDVNTD FLLILLKGLQ
541RLNPALRLVL MSATGDNERF SRYFGGCPVI KVPGFMYPVK EHYLEDILAK LGKHQYLHRH
601RHHESEDECA LDLDLVTDLV LHIDARGEPG GILCFLPGWQ EIKGVQQRLQ EALGMHESKY
661LILPVHSNIP MMDQKAIFQQ PPVGVRKIVL ATNIAETSIT INDIVHVVDS GLHKEERYDL
721KTKVSCLETV WVSRANVIQR RGRAGRCQSG FAYHLFPRSR LEKMVPFQVP EILRTPLENL
781VLQAKIHMPE KTAVEFLSKA VDSPNIKAVD EAVILLQEIG VLDQREYLTT LGQRLAHIST
841DPRLAKAIVL AAIFRCLHPL LVVVSCLTRD PFSSSLQNRA EVDKVKALLS HDSGSDHLAF
901VRAVAGWEEV LRWQDRSSRE NYLEENLLYA PSLRFIHGLI KQFSENIYEA FLVGKPSDCT
961LASAQCNEYS EEEELVKGVL MAGLYPNLIQ VRQGKVTRQG KFKPNSVTYR TKSGNILLHK
1021STINREATRL RSRWLTYFMA VKSNGSVFVR DSSQVHPLAV LLLTDGDVHI RDDGRRATIS
1081LSDSDLLRLE GDSRTVRLLK ELRRALGRMV ERSLRSELAA LPPSVQEEHG QLLALLAELL
1141RGPCGSFDVR KTADD
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1155 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.