DENND5B Antibody (aa1066-1094)
Catalog Number: LS-C167362-400
400 µL / $401.00
Antibody: Rabbit Polyclonal
Applications: Immunohistochemistry (general), Immunohistochemistry, Paraffin, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DENND5B Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G95005-20
Catalog Number: LS-G95005
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DENND5B (NM_144973) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DENN/MADD domain containing 5B | DENND5B | Rab6IP1-like protein
- Origin Species
- Human
- NCBI GenBank
- NM_144973
- SwissProt
- Q6ZUT9
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 144.8 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGSCAAPGP GSGSSPAACR FAHYFVLCGI DADSGLEPDE LAGENFDQSP LRRTFKSKVL61AHYPQNIEWN PFDQDAVNML CMPKGLSFRT QTDNKDPQFH SFIITREDGS RTYGFVLTFY121EEVTSKQICT AMQTLYQMHN AEHYSSVYAS SSCSMDSLAS SLDEGDTTSL LKLQRYNSYD181ISRDTLYVSK SICLITPLPF MQACKKFLIQ LYKAVTSQQP PPLPLESYIH NILYEVPLPP241PGRSLKFYGV YEPVICQRPG PSELPLSDYP LREAFELLGL ENLVQVFTCV LLEMQILLYS301QDYQRLMTVA EGITTLLFPF QWQHVYVPIL PASLLHFLDA PVPYLMGLQS KEGTDRSKLE361LPQEANLCFV DIDNHFIELP EEFPQFPNKV DFIQELSEVL VQFGIPPEGS LHCSESTSKL421KNMVLKDLVN DKKNGNVCTN NISMYELLKG NETIARLQAL AKRTGVAVEK MDLSASLGEK481DKDLKLHCEE AELRDYQLNV QLREVFANRF TQMFADYEAF VIQTAQDMES WLTNREQMQN541FDKASFLSDQ PEPYLPFLSR FIETQMFATF IDNKIMSQWE EKDPLLRVFD TRIDKIRLYN601VRAPTLRTSI YQKCSTLKEA AQSIEQRLMK MDHTAIHPHL LDMKIGQGKY EQGFFPKLQS661DVLATGPTSN NRWVSRSATA QRRKERLRQH SEHVGLDNDL REKYMQEARS LGKNLRQPKL721SDLSPAVIAQ TNCKFVEGLL KECRMKTKRM LVEKMGHEAV ELGHGEANIT GLEENTLIAS781LCDLLERIWS HGLQVKQGKS ALWSHLIQFQ DREEKQEHLA ESPVALGPER RKSDSGVMLP841TLRVSLIQDM RHIQNMSEIK TDVGRARAWI RLSLEKKLLS QHLKQLLSNQ PLTKKLYKRY901AFLRCEEERE QFLYHLLSLN AVDYFCFTSV FTTIMIPYRS VIIPIKKLSN AIITSNPWIC961VSGELGDTGV MQIPKNLLEM TFECQNLGKL TTVQIGHDNS GLLAKWLVDC VMVRNEITGH1021TYRFPCGRWL GKGIDDGSLE RILIGELMTS ASDEDLVKQC RTPPQQKSPT TARRLSITSL1081TGKNNKPNAG QIQEGIGEAV NNIVKHFHKP EKERGSLTVL LCGENGLVAA LEQVFHHGFK1141SARIFHKNVF IWDFIEKVVA YFETTDQILD NEDDVLIQKS SCKTFCHYVN AINTAPRNIG1201KDGKFQILVC LGTRDRLLPQ WIPLLAECPA ITRMYEESAL LRDRMTVNSL IRILQTIQDF1261TIVLEGSLIK GVDVTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1274 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
