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DENND5B Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DENND5B Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DENND5B antibodies →
$725.00each
Catalog Number: LS-G106828-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENND5B (NM_144973), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DENN/MADD domain containing 5B | DENND5B | Rab6IP1-like protein
Origin Species
Human
NCBI GenBank
NM_144973
SwissProt
Q6ZUT9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
144.8 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGSCAAPGP GSGSSPAACR FAHYFVLCGI DADSGLEPDE LAGENFDQSP LRRTFKSKVL
61AHYPQNIEWN PFDQDAVNML CMPKGLSFRT QTDNKDPQFH SFIITREDGS RTYGFVLTFY
121EEVTSKQICT AMQTLYQMHN AEHYSSVYAS SSCSMDSLAS SLDEGDTTSL LKLQRYNSYD
181ISRDTLYVSK SICLITPLPF MQACKKFLIQ LYKAVTSQQP PPLPLESYIH NILYEVPLPP
241PGRSLKFYGV YEPVICQRPG PSELPLSDYP LREAFELLGL ENLVQVFTCV LLEMQILLYS
301QDYQRLMTVA EGITTLLFPF QWQHVYVPIL PASLLHFLDA PVPYLMGLQS KEGTDRSKLE
361LPQEANLCFV DIDNHFIELP EEFPQFPNKV DFIQELSEVL VQFGIPPEGS LHCSESTSKL
421KNMVLKDLVN DKKNGNVCTN NISMYELLKG NETIARLQAL AKRTGVAVEK MDLSASLGEK
481DKDLKLHCEE AELRDYQLNV QLREVFANRF TQMFADYEAF VIQTAQDMES WLTNREQMQN
541FDKASFLSDQ PEPYLPFLSR FIETQMFATF IDNKIMSQWE EKDPLLRVFD TRIDKIRLYN
601VRAPTLRTSI YQKCSTLKEA AQSIEQRLMK MDHTAIHPHL LDMKIGQGKY EQGFFPKLQS
661DVLATGPTSN NRWVSRSATA QRRKERLRQH SEHVGLDNDL REKYMQEARS LGKNLRQPKL
721SDLSPAVIAQ TNCKFVEGLL KECRMKTKRM LVEKMGHEAV ELGHGEANIT GLEENTLIAS
781LCDLLERIWS HGLQVKQGKS ALWSHLIQFQ DREEKQEHLA ESPVALGPER RKSDSGVMLP
841TLRVSLIQDM RHIQNMSEIK TDVGRARAWI RLSLEKKLLS QHLKQLLSNQ PLTKKLYKRY
901AFLRCEEERE QFLYHLLSLN AVDYFCFTSV FTTIMIPYRS VIIPIKKLSN AIITSNPWIC
961VSGELGDTGV MQIPKNLLEM TFECQNLGKL TTVQIGHDNS GLLAKWLVDC VMVRNEITGH
1021TYRFPCGRWL GKGIDDGSLE RILIGELMTS ASDEDLVKQC RTPPQQKSPT TARRLSITSL
1081TGKNNKPNAG QIQEGIGEAV NNIVKHFHKP EKERGSLTVL LCGENGLVAA LEQVFHHGFK
1141SARIFHKNVF IWDFIEKVVA YFETTDQILD NEDDVLIQKS SCKTFCHYVN AINTAPRNIG
1201KDGKFQILVC LGTRDRLLPQ WIPLLAECPA ITRMYEESAL LRDRMTVNSL IRILQTIQDF
1261TIVLEGSLIK GVDV
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1274 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.