DENND5A Antibody
Catalog Number: LS-C399288
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Immunohistochemistry, Paraffin
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DENND5A Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G83162-20
Catalog Number: LS-G83162
For bulk orders or custom formulations:
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DENND5A (NM_015213) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DENN/MADD domain containing 5A | RAB6IP1 | RAB6 interacting protein 1 | DENND5A | KIAA1091 | Rab6-interacting protein 1
- Origin Species
- Human
- NCBI GenBank
- NM_015213
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 146.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG ASPFISSTTE61GENFEQTPLR RTFKSKVLAR YPENVEWNPF DQDAVGMLCM PKGLAFKTQA DPREPQFHAF121IITREDGSRT FGFALTFYEE VTSKQICSAM QTLYHMHNAE YDVLHAPPAD DRDQSSMEDG181EDTPVTKLQR FNSYDISRDT LYVSKCICLI TPMSFMKACR SVLQQLHQAV TSPQPPPLPL241ESYIYNVLYE VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ301LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL HFLDAPVPYL361MGLHSNGLDD RSKLELPQEA NLCFVDIDNH FIELPEDLPQ FPNKLEFVQE VSEILMAFGI421PPEGNLHCSE SASKLKRLRA SELVSDKRNG NIAGSPLHSY ELLKENETIA RLQALVKRTG481VSLEKLEVRE DPSSNKDLKV QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ541DKESWFTNRE QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV601LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA IHPHLLDMKI661GQGKYEPGFF PKLQSDVLST GPASNKWTKR NAPAQWRRKD RQKQHTEHLR LDNDQREKYI721QEARTMGSTI RQPKLSNLSP SVIAQTNWKF VEGLLKECRN KTKRMLVEKM GREAVELGHG781EVNITGVEEN TLIASLCDLL ERIWSHGLQV KQGKSALWSH LLHYQDNRQR KLTSGSLSTS841GILLDSERRK SDASSLMPPL RISLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH901LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT TILIPYHILI961VPSKKLGGSM FTANPWICIS GELGETQIMQ IPRNVLEMTF ECQNLGKLTT VQIGHDNSGL1021YAKWLVEYVM VRNEITGHTY KFPCGRWLGK GMDDGSLERI LVGELLTSQP EVDERPCRTP1081PLQQSPSVIR RLVTISPNNK PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE1141CGLVSALEQA FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EKNEVVPEEN WHTRARNFCR1201FVTAINNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED VALIKDHTLV1261NSLIRVLQTL QEFNITLETS LVKGIDITRTRPLEQKLISEEDLAANDILDYKDDDDKV
1287 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
