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DENND5A Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DENND5A Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DENND5A antibodies →
$504.00each
Catalog Number: LS-G101299-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENND5A (NM_015213), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DENN/MADD domain containing 5A | RAB6IP1 | RAB6 interacting protein 1 | DENND5A | KIAA1091 | Rab6-interacting protein 1
Origin Species
Human
NCBI GenBank
NM_015213

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
146.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSGGGGGGGS APSRFADYFV ICGLDTETGL EPDELSALCQ YIQASKARDG ASPFISSTTE
61GENFEQTPLR RTFKSKVLAR YPENVEWNPF DQDAVGMLCM PKGLAFKTQA DPREPQFHAF
121IITREDGSRT FGFALTFYEE VTSKQICSAM QTLYHMHNAE YDVLHAPPAD DRDQSSMEDG
181EDTPVTKLQR FNSYDISRDT LYVSKCICLI TPMSFMKACR SVLQQLHQAV TSPQPPPLPL
241ESYIYNVLYE VPLPPPGRSL KFSGVYGPII CQRPSTNELP LFDFPVKEVF ELLGVENVFQ
301LFTCALLEFQ ILLYSQHYQR LMTVAETITA LMFPFQWQHV YVPILPASLL HFLDAPVPYL
361MGLHSNGLDD RSKLELPQEA NLCFVDIDNH FIELPEDLPQ FPNKLEFVQE VSEILMAFGI
421PPEGNLHCSE SASKLKRLRA SELVSDKRNG NIAGSPLHSY ELLKENETIA RLQALVKRTG
481VSLEKLEVRE DPSSNKDLKV QCDEEELRIY QLNIQIREVF ANRFTQMFAD YEVFVIQPSQ
541DKESWFTNRE QMQNFDKASF LSDQPEPYLP FLSRFLETQM FASFIDNKIM CHDDDDKDPV
601LRVFDSRVDK IRLLNVRTPT LRTSMYQKCT TVDEAEKAIE LRLAKIDHTA IHPHLLDMKI
661GQGKYEPGFF PKLQSDVLST GPASNKWTKR NAPAQWRRKD RQKQHTEHLR LDNDQREKYI
721QEARTMGSTI RQPKLSNLSP SVIAQTNWKF VEGLLKECRN KTKRMLVEKM GREAVELGHG
781EVNITGVEEN TLIASLCDLL ERIWSHGLQV KQGKSALWSH LLHYQDNRQR KLTSGSLSTS
841GILLDSERRK SDASSLMPPL RISLIQDMRH IQNIGEIKTD VGKARAWVRL SMEKKLLSRH
901LKQLLSDHEL TKKLYKRYAF LRCDDEKEQF LYHLLSFNAV DYFCFTNVFT TILIPYHILI
961VPSKKLGGSM FTANPWICIS GELGETQIMQ IPRNVLEMTF ECQNLGKLTT VQIGHDNSGL
1021YAKWLVEYVM VRNEITGHTY KFPCGRWLGK GMDDGSLERI LVGELLTSQP EVDERPCRTP
1081PLQQSPSVIR RLVTISPNNK PKLNTGQIQE SIGEAVNGIV KHFHKPEKER GSLTLLLCGE
1141CGLVSALEQA FQHGFKSPRL FKNVFIWDFL EKAQTYYETL EKNEVVPEEN WHTRARNFCR
1201FVTAINNTPR NIGKDGKFQM LVCLGARDHL LHHWIALLAD CPITAHMYED VALIKDHTLV
1261NSLIRVLQTL QEFNITLETS LVKGIDI
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1287 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.