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Human DENND2A / KIAA1277 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DENND2A / KIAA1277 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DENND2A antibodies →
$220.00each
Catalog Number: LS-G86385-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DENND2A (NM_015689) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DENN/MADD domain containing 2A | DENND2A | FAM31D | KIAA1277
Origin Species
Human
NCBI GenBank
AB033103
SwissProt
Q9ULE3

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
113.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MDMFSLDMII SDPAAEASRA GKKQLRGVQN PCPSARARPR HKSLNIKDKI SEWEGKKEVP
61TPAPSRRADG QEDYLPSSTV ERRSSDGVRT QVTEAKNGMR PGTESTEKER NKGAVNVGGQ
121DPEPGQDLSQ PEREVDPSWG RGREPRLGKL RFQNDHLSVL KQVKKLEQAL KDGSAGLDPQ
181LPGTCYSPHC PPDKAEAGST LPENLGGGSG SEVSQRVHPS DLEGREPTPE LVEDRKGSCR
241RPWDRSLENV YRGSEGSPTK PFINPLPKPR RTFKHAGEGD KDGKPGIGFR KEKRNLPPLP
301SLPPPPLPSS PPPSSVNRRL WTGRQKSSAD HRKSYEFEDL LQSSSESSRV DWYAQTKLGL
361TRTLSEENVY EDILDPPMKE NPYEDIELHG RCLGKKCVLN FPASPTSSIP DTLTKQSLSK
421PAFFRQNSER RNFKLLDTRK LSRDGTGSPS KISPPSTPSS PDDIFFNLGD PQNGRKKRKI
481PKLVLRINAI YEVRRGKKRV KRLSQSMESN SGKVTDENSE SDSDTEEKLK AHSQRLVNVK
541SRLKQAPRYP SLARELIEYQ ERQLFEYFVV VSLHKKQAGA AYVPELTQQF PLKLERSFKF
601MREAEDQLKA IPQFCFPDAK DWVPVQQFTS ETFSFVLTGE DGSRRFGYCR RLLPGGKGKR
661LPEVYCIVSR LGCFSLFSRI LDEVEKRRGI SPALVQPLMR SVMEAPFPAL GKTILVKNFL
721PGSGTEVIEL CRPLDSRLEH VDFESLFSSL SVRHLVCVFA SLLLERRVIF IADKLSILSK
781CCHAMVALIY PFAWQHTYIP VLPPAMVDIV CSPTPFLIGL LSSSLPLLRE LPLEEVLVVD
841LVNSRFLRQM DDEDSILPRK LQVALEHILE QRNELACEQD EGPLDGRHGP ESSPLNEVVS
901EAFVRFFVEI VGHYSLFLTS GEREERTLQR EAFRKAVSSK SLRHFLEVFM ETQMFRGFIQ
961ERELRRQDAK GLFEVRAQEY LETLPSGEHS GVNKFLKGLG NKMKFLHKK
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

1009 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.