Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DENND2A / KIAA1277 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G113054-100
Catalog Number: LS-G113054
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENND2A (NM_015689), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DENN/MADD domain containing 2A | DENND2A | FAM31D | KIAA1277
- Origin Species
- Human
- NCBI GenBank
- AB033103
- SwissProt
- Q9ULE3
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 113.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MDMFSLDMII SDPAAEASRA GKKQLRGVQN PCPSARARPR HKSLNIKDKI SEWEGKKEVP61TPAPSRRADG QEDYLPSSTV ERRSSDGVRT QVTEAKNGMR PGTESTEKER NKGAVNVGGQ121DPEPGQDLSQ PEREVDPSWG RGREPRLGKL RFQNDHLSVL KQVKKLEQAL KDGSAGLDPQ181LPGTCYSPHC PPDKAEAGST LPENLGGGSG SEVSQRVHPS DLEGREPTPE LVEDRKGSCR241RPWDRSLENV YRGSEGSPTK PFINPLPKPR RTFKHAGEGD KDGKPGIGFR KEKRNLPPLP301SLPPPPLPSS PPPSSVNRRL WTGRQKSSAD HRKSYEFEDL LQSSSESSRV DWYAQTKLGL361TRTLSEENVY EDILDPPMKE NPYEDIELHG RCLGKKCVLN FPASPTSSIP DTLTKQSLSK421PAFFRQNSER RNFKLLDTRK LSRDGTGSPS KISPPSTPSS PDDIFFNLGD PQNGRKKRKI481PKLVLRINAI YEVRRGKKRV KRLSQSMESN SGKVTDENSE SDSDTEEKLK AHSQRLVNVK541SRLKQAPRYP SLARELIEYQ ERQLFEYFVV VSLHKKQAGA AYVPELTQQF PLKLERSFKF601MREAEDQLKA IPQFCFPDAK DWVPVQQFTS ETFSFVLTGE DGSRRFGYCR RLLPGGKGKR661LPEVYCIVSR LGCFSLFSRI LDEVEKRRGI SPALVQPLMR SVMEAPFPAL GKTILVKNFL721PGSGTEVIEL CRPLDSRLEH VDFESLFSSL SVRHLVCVFA SLLLERRVIF IADKLSILSK781CCHAMVALIY PFAWQHTYIP VLPPAMVDIV CSPTPFLIGL LSSSLPLLRE LPLEEVLVVD841LVNSRFLRQM DDEDSILPRK LQVALEHILE QRNELACEQD EGPLDGRHGP ESSPLNEVVS901EAFVRFFVEI VGHYSLFLTS GEREERTLQR EAFRKAVSSK SLRHFLEVFM ETQMFRGFIQ961ERELRRQDAK GLFEVRAQEY LETLPSGEHS GVNKFLKGLG NKMKFLHKKTRTRPLEQKLISEEDLAANDILDYKDDDDKV
1009 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.