DENND1C Antibody (HRP)
Catalog Number: LS-C677663
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DENND1C Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G84586-20
Catalog Number: LS-G84586
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DENND1C (NM_024898) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- Connecdenn 3 | DENN/MADD domain containing 1C | DENND1C | FAM31C | Protein FAM31C
- Origin Species
- Human
- NCBI GenBank
- NM_024898
- SwissProt
- Q8IV53
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 86.9 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MESRAEGGSP AVFDWFFEAA CPASLQEDPP ILRQFPPDFR DQEAMQMVPK FCFPFDVERE61PPSPAVQHFT FALTDLAGNR RFGFCRLRAG TQSCLCILSH LPWFEVFYKL LNTVGDLLAQ121DQVTEAEELL QNLFQQSLSG PQASVGLELG SGVTVSSGQG IPPPTRGNSK PLSCFVAPDS181GRLPSIPENR NLTELVVAVT DENIVGLFAA LLAERRVLLT ASKLSTLTSC VHASCALLYP241MRWEHVLIPT LPPHLLDYCC APMPYLIGVH ASLAERVREK ALEDVVVLNV DANTLETTFN301DVQALPPDVV SLLRLRLRKV ALAPGEGVSR LFLKAQALLF GGYRDALVCS PGQPVTFSEE361VFLAQKPGAP LQAFHRRAVH LQLFKQFIEA RLEKLNKGEG FSDQFEQEIT GCGASSGALR421SYQLWADNLK KGGGALLHSV KAKTQPAVKN MYRSAKSGLK GVQSLLMYKD GDSVLQRGGS481LRAPALPSRS DRLQQRLPIT QHFGKNRPLR PSRRRQLEEG TSEPPGAGTP PLSPEDEGCP541WAEEALDSSF LGSGEELDLL SEILDSLSMG AKSAGSLRPS QSLDCCHRGD LDSCFSLPNI601PRWQPDDKKL PEPEPQPLSL PSLQNASSLD ATSSSKDSRS QLIPSESDQE VTSPSQSSTA661SADPSIWGDP KPSPLTEPLI LHLTPSHKAA EDSTAQENPT PWLSTAPTEP SPPESPQILA721PTKPNFDIAW TSQPLDPSSD PSSLEDPRAR PPKALLAERA HLQPREEPGA LNSPATPTSN781CQKSQPSSRP RVADLKKCFE GSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
801 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
