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DENND1C Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DENND1C Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DENND1C antibodies →
$504.00each
Catalog Number: LS-G109690-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DENND1C (NM_024898), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
Connecdenn 3 | DENN/MADD domain containing 1C | DENND1C | FAM31C | Protein FAM31C
Origin Species
Human
NCBI GenBank
NM_024898
SwissProt
Q8IV53

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
86.9 kDa
Protein Type
Lysate
Amino Acid Sequence
1MESRAEGGSP AVFDWFFEAA CPASLQEDPP ILRQFPPDFR DQEAMQMVPK FCFPFDVERE
61PPSPAVQHFT FALTDLAGNR RFGFCRLRAG TQSCLCILSH LPWFEVFYKL LNTVGDLLAQ
121DQVTEAEELL QNLFQQSLSG PQASVGLELG SGVTVSSGQG IPPPTRGNSK PLSCFVAPDS
181GRLPSIPENR NLTELVVAVT DENIVGLFAA LLAERRVLLT ASKLSTLTSC VHASCALLYP
241MRWEHVLIPT LPPHLLDYCC APMPYLIGVH ASLAERVREK ALEDVVVLNV DANTLETTFN
301DVQALPPDVV SLLRLRLRKV ALAPGEGVSR LFLKAQALLF GGYRDALVCS PGQPVTFSEE
361VFLAQKPGAP LQAFHRRAVH LQLFKQFIEA RLEKLNKGEG FSDQFEQEIT GCGASSGALR
421SYQLWADNLK KGGGALLHSV KAKTQPAVKN MYRSAKSGLK GVQSLLMYKD GDSVLQRGGS
481LRAPALPSRS DRLQQRLPIT QHFGKNRPLR PSRRRQLEEG TSEPPGAGTP PLSPEDEGCP
541WAEEALDSSF LGSGEELDLL SEILDSLSMG AKSAGSLRPS QSLDCCHRGD LDSCFSLPNI
601PRWQPDDKKL PEPEPQPLSL PSLQNASSLD ATSSSKDSRS QLIPSESDQE VTSPSQSSTA
661SADPSIWGDP KPSPLTEPLI LHLTPSHKAA EDSTAQENPT PWLSTAPTEP SPPESPQILA
721PTKPNFDIAW TSQPLDPSSD PSSLEDPRAR PPKALLAERA HLQPREEPGA LNSPATPTSN
781CQKSQPSSRP RVADLKKCFE G
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

801 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.