DEF8 Antibody (HRP)
Catalog Number: LS-C398837
2 sizes / from $300.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DEF8 Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83832-20
Catalog Number: LS-G83832
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DEF8 (NM_207514) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DEF-8 | FLJ20186 | DEF8
- Origin Species
- Human
- NCBI GenBank
- NM_017702
- SwissProt
- Q6ZN54
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 58.5 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAILSLRAPG PWQAMQVWAD RTLLTPHTGV TSQVLGVAAA VMTPLPGGHA AGRTREARWD61AMEYDEKLAR FRQAHLNPFN KQSGPRQHEQ GPGEEVPDVT PEEALPELPP GEPEFRCPER121VMDLGLSEDH FSRPVGLFLA SDVQQLRQAI EECKQVILEL PEQSEKQKDA VVRLIHLRLK181LQELKDPNED EPNIRVLLEH RFYKEKSKSV KQTCDKCNTI IWGLIQTWYT CTGCYYRCHS241KCLNLISKPC VSSKVSHQAE YELNICPETG LDSQDYRCAE CRAPISLRGV PSEARQCDYT301GQYYCSHCHW NDLAVIPARV VHNWDFEPRK VSRCSMRYLA LMVSRPVLRL REINPLLFSY361VEELVEIRKL RQDILLMKPY FITCREAMEA RLLLQLQDRQ HFVENDEMYS VQDLLDVHAG421RLGCSLTEIH TLFAKHIKLD CERCQAKGFV CELCREGDVL FPFDSHTSVC ADCSAVFHRD481CYYDNSTTCP KCARLSLRKQ SLFQEPGPDV EASGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
512 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
