LSBio
DEF8 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DEF8 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DEF8 antibodies →
$725.00each
Catalog Number: LS-G102607-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DEF8 (NM_207514), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DEF-8 | FLJ20186 | DEF8
Origin Species
Human
NCBI GenBank
NM_017702
SwissProt
Q6ZN54

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
58.5 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAILSLRAPG PWQAMQVWAD RTLLTPHTGV TSQVLGVAAA VMTPLPGGHA AGRTREARWD
61AMEYDEKLAR FRQAHLNPFN KQSGPRQHEQ GPGEEVPDVT PEEALPELPP GEPEFRCPER
121VMDLGLSEDH FSRPVGLFLA SDVQQLRQAI EECKQVILEL PEQSEKQKDA VVRLIHLRLK
181LQELKDPNED EPNIRVLLEH RFYKEKSKSV KQTCDKCNTI IWGLIQTWYT CTGCYYRCHS
241KCLNLISKPC VSSKVSHQAE YELNICPETG LDSQDYRCAE CRAPISLRGV PSEARQCDYT
301GQYYCSHCHW NDLAVIPARV VHNWDFEPRK VSRCSMRYLA LMVSRPVLRL REINPLLFSY
361VEELVEIRKL RQDILLMKPY FITCREAMEA RLLLQLQDRQ HFVENDEMYS VQDLLDVHAG
421RLGCSLTEIH TLFAKHIKLD CERCQAKGFV CELCREGDVL FPFDSHTSVC ADCSAVFHRD
481CYYDNSTTCP KCARLSLRKQ SLFQEPGPDV EA
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

512 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.