DEF6 Antibody
Catalog Number: LS-C749341
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DEF6 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G91569-20
Catalog Number: LS-G91569
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DEF6 (NM_022047) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DEF6 | DEF-6 | IBP | SLAT | SWAP70L | IRF4-binding protein
- Origin Species
- Human
- NCBI GenBank
- NM_022047
- SwissProt
- Q9H4E7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 73.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALRKELLKS IWYAFTALDV EKSGKVSKSQ LKVLSHNLYT VLHIPHDPVA LEEHFRDDDD61GPVSSQGYMP YLNKYILDKV EEGAFVKEHF DELCWTLTAK KNYRADSNGN SMLSNQDAFR121LWCLFNFLSE DKYPLIMVPD EVEYLLKKVL SSMSLEVSLG ELEELLAQEA QVAQTTGGLS181VWQFLELFNS GRCLRGVGRD TLSMAIHEVY QELIQDVLKQ GYLWKRGHLR RNWAERWFQL241QPSCLCYFGS EECKEKRGII PLDAHCCVEV LPDRDGKRCM FCVKTATRTY EMSASDTRQR301QEWTAAIQMA IRLQAEGKTS LHKDLKQKRR EQREQRERRR AAKEEELLRL QQLQEEKERK361LQELELLQEA QRQAERLLQE EEERRRSQHR ELQQALEGQL REAEQARASM QAEMELKEEE421AARQRQRIKE LEEMQQRLQE ALQLEVKARR DEESVRIAQT RLLEEEEEKL KQLMQLKEEQ481ERYIERAQQE KEELQQEMAQ QSRSLQQAQQ QLEEVRQNRQ RADEDVEAAQ RKLRQASTNV541KHWNVQMNRL MHPIEPGDKR PVTSSSFSGF QPPLLAHRDS SLKRLTRWGS QGNRTPSPNS601NEQQKSLNGG DEAPAPASTP QEDKLDPAPE NTRTRPLEQKLISEEDLAANDILDYKDDDDKV
631 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
