DDX55 Antibody
Catalog Number: LS-C804838
2 sizes / from $288.00
Antibody: Rabbit Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot
Reactivity: Human, Mouse
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DDX55 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G87285-20
Catalog Number: LS-G87285
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DDX55 (NM_020936) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDX55 | KIAA1595 | DEAD box protein 55
- Origin Species
- Human
- NCBI GenBank
- NM_020936
- SwissProt
- Q8NHQ9
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 68.4 kda
- Protein Type
- Lysate
- Amino Acid Sequence
1MEHVTEGSWE SLPVPLHPQV LGALRELGFP YMTPVQSATI PLFMRNKDVA AEAVTGSGKT61LAFVIPILEI LLRREEKLKK SQVGAIIITP TRELAIQIDE VLSHFTKHFP EFSQILWIGG121RNPGEDVERF KQQGGNIIVA TPGRLEDLFR RKAEGLDLAS CVRSLDVLVL DEADRLLDMG181FEASINTILE FLPKQRRTGL FSATQTQEVE NLVRAGLRNP VRVSVKEKGV AASSAQKTPS241RLENYYMVCK ADEKFNQLVH FLRNHKQEKH LVFFSTCACV EYYGKTLEVL VKGVKIMCIH301GKMKYKRNKI FMEFRKLQSG ILVCTDVMAR GIDIPEVNWV LQYDPPSNAS AFVHRCGRTA361RIGHGGSALV FLLPMEESYI NFLAINQKCP LQEMKPQRNT ADLLPKLKSM ALADRAVFEK421GMKAFVSYVQ AYAKHECNLI FRLKDLDFAS LARGFALLRM PKMPELRGKQ FPDFVPVDVN481TDTIPFKDKI REKQRQKLLE QQRREKTENE GRRKFIKNKA WSKQKAKKEK KKKMNEKRKR541EEGSDIEDED MEELLNDTRL LKKLKKGKIT EEEFEKGLLT TGKRTIKTVD LGISDLEDGCTRTRPLEQKLISEEDLAANDILDYKDDDDKV
600 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
