LSBio
DDX55 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDX55 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DDX55 antibodies →
$504.00each
Catalog Number: LS-G110872-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX55 (NM_020936), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX55 | KIAA1595 | DEAD box protein 55
Origin Species
Human
NCBI GenBank
NM_020936
SwissProt
Q8NHQ9

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
68.4 kda
Protein Type
Lysate
Amino Acid Sequence
1MEHVTEGSWE SLPVPLHPQV LGALRELGFP YMTPVQSATI PLFMRNKDVA AEAVTGSGKT
61LAFVIPILEI LLRREEKLKK SQVGAIIITP TRELAIQIDE VLSHFTKHFP EFSQILWIGG
121RNPGEDVERF KQQGGNIIVA TPGRLEDLFR RKAEGLDLAS CVRSLDVLVL DEADRLLDMG
181FEASINTILE FLPKQRRTGL FSATQTQEVE NLVRAGLRNP VRVSVKEKGV AASSAQKTPS
241RLENYYMVCK ADEKFNQLVH FLRNHKQEKH LVFFSTCACV EYYGKTLEVL VKGVKIMCIH
301GKMKYKRNKI FMEFRKLQSG ILVCTDVMAR GIDIPEVNWV LQYDPPSNAS AFVHRCGRTA
361RIGHGGSALV FLLPMEESYI NFLAINQKCP LQEMKPQRNT ADLLPKLKSM ALADRAVFEK
421GMKAFVSYVQ AYAKHECNLI FRLKDLDFAS LARGFALLRM PKMPELRGKQ FPDFVPVDVN
481TDTIPFKDKI REKQRQKLLE QQRREKTENE GRRKFIKNKA WSKQKAKKEK KKKMNEKRKR
541EEGSDIEDED MEELLNDTRL LKKLKKGKIT EEEFEKGLLT TGKRTIKTVD LGISDLEDGC
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

600 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.