DDX51 Antibody
Catalog Number: LS-C750550
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DDX51 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G92429-20
Catalog Number: LS-G92429
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DDX51 (NM_175066) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDX51 | DEAD box protein 51
- Origin Species
- Human
- NCBI GenBank
- BC012461
- SwissProt
- Q8N8A6
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 72.3 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MALFYVARYP GPDAAAAAGP EGAEAGAHGR ARALLERLQS RARERQQQRE PAQTEAAAST61EPATRRRRRP RRRRRVNDAE PGSPEAPQGK RRKADGEDAG AESNEEAPGE PSAGSSEEAP121GEPSAGSSEE APGERSTSAS AEAAPDGPAL EEAAGPLVPG LVLGGFGKRK APKVQPFLPR181WLAEPNCVRR NVTEDLVPIE DIPDVHPDLQ KQLRAHGISS YFPVQAAVIP ALLESAACGF241LVGRGGYRPS DLCVSAPTGS GKTLAFVIPV VQALLSRVVC HIRALVVLPT KELAQQVSKV301FNIYTDATPL RVSLVTGQKS LAKEQESLVQ KTADGYRCLA DIVVATPGRL VDHIDQTPGF361SLQQLRFLII DEADRMIDSM HQSWLPRVVA AAFQSEDPAD PCALLQRRQA QAVTAASTCC421PQMPLQKLLF SATLTQNPEK LQQLGLHQPR LFSTGLAHRG LEDTDGDGDS GKYAFPVGLT481HHYVPCSLSS KPLVVLHLVL EMGFSRVLCF TNSRENSHRL FLLVQAFGGV DVAEFSSRYG541PGQRRMILKQ FEQGKIQLLI STDATARGID VQGVELVVNY DAPQYLRTYV HRVGRTARAG601KTGQAFTLLL KVQERRFLRM LTEAGAPELQ RHELSSKLLQ PLVPRYEEAL SQLEESVKEE661RKQRAATRTRPLEQKLISEEDLAANDILDYKDDDDKV
666 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
