LSBio
DDX51 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDX51 Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DDX51 antibodies →
$504.00each
Catalog Number: LS-G105047-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX51 (NM_175066), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX51 | DEAD box protein 51
Origin Species
Human
NCBI GenBank
BC012461
SwissProt
Q8N8A6

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
72.3 kDa
Protein Type
Lysate
Amino Acid Sequence
1MALFYVARYP GPDAAAAAGP EGAEAGAHGR ARALLERLQS RARERQQQRE PAQTEAAAST
61EPATRRRRRP RRRRRVNDAE PGSPEAPQGK RRKADGEDAG AESNEEAPGE PSAGSSEEAP
121GEPSAGSSEE APGERSTSAS AEAAPDGPAL EEAAGPLVPG LVLGGFGKRK APKVQPFLPR
181WLAEPNCVRR NVTEDLVPIE DIPDVHPDLQ KQLRAHGISS YFPVQAAVIP ALLESAACGF
241LVGRGGYRPS DLCVSAPTGS GKTLAFVIPV VQALLSRVVC HIRALVVLPT KELAQQVSKV
301FNIYTDATPL RVSLVTGQKS LAKEQESLVQ KTADGYRCLA DIVVATPGRL VDHIDQTPGF
361SLQQLRFLII DEADRMIDSM HQSWLPRVVA AAFQSEDPAD PCALLQRRQA QAVTAASTCC
421PQMPLQKLLF SATLTQNPEK LQQLGLHQPR LFSTGLAHRG LEDTDGDGDS GKYAFPVGLT
481HHYVPCSLSS KPLVVLHLVL EMGFSRVLCF TNSRENSHRL FLLVQAFGGV DVAEFSSRYG
541PGQRRMILKQ FEQGKIQLLI STDATARGID VQGVELVVNY DAPQYLRTYV HRVGRTARAG
601KTGQAFTLLL KVQERRFLRM LTEAGAPELQ RHELSSKLLQ PLVPRYEEAL SQLEESVKEE
661RKQRAA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

666 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.