DDX31 Antibody
Catalog Number: LS-C833617
3 sizes / from $338.00
Antibody: Rabbit Polyclonal
Applications: Immunofluorescence, Western Blot
Reactivity: Human, Mouse, Rat
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DDX31 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$220.00each
Catalog Number: LS-G83878-20
Catalog Number: LS-G83878
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DDX31 (NM_138620) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DDX31 | DEAD box protein 31 | DEAD/DEXH helicase DDX31 | G2 helicase | Helicain | PPP1R25
- Origin Species
- Human
- NCBI GenBank
- NM_022779
- SwissProt
- Q9H8H2
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 64 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MAPDLASQRH SESFPSVNSR PNVILPGREG RREGLPPGGG TRGSLVPTRP VPPSPAPLGT61SPYSWSRSGP GRGGGAGSSR VPRGVPGPAV CAPGSLLHHA SPTQTMAAAD GSLFDNPRTF121SRRPPAQASR QAKATKRKYQ ASSEAPPAKR RNETSFLPAK KTSVKETQRT FKGNAQKMFS181PKKHSVSTSD RNQEERQCIK TSSLFKNNPD IPELHRPVVK QVQEKVFTSA AFHELGLHPH241LISTINTVLK MSSMTSVQKQ SIPVLLEGRD ALVRSQTGSG KTLAYCIPVV QSLQAMESKI301QRSDGPYALV LVPTRELALQ SFDTVQKLLK PFTWIVPGVL MGGEKRKSEK ARLRKGINIL361ISTPGRLVDH IKSTKNIHFS RLRWLVFDEA DRILDLGFEK DITVILNAVN AECQKRQNVL421LSATLTEGVT RLADISLHDP VSISVLDKSH DQLNPKDKAV QEVCPPPAGD KLDSFAIPES481LKQHVTVVPS KLRLVCLAAF ILQKCKFEED QKMVVFFSSC ELVEFHYSLF LQTLLSSSGA541PASGQLPSAS MRLKFLRLHG GMEQEERTAV FQEFSHSRRG VLLCTSGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
585 aa + 34 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
