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DDX31 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DDX31 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293
Format:Liquid
See all DDX31 antibodies →
$725.00each
Catalog Number: LS-G107165-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DDX31 (NM_138620), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DDX31 | DEAD box protein 31 | DEAD/DEXH helicase DDX31 | G2 helicase | Helicain | PPP1R25
Origin Species
Human
NCBI GenBank
NM_022779
SwissProt
Q9H8H2

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
64 kDa
Protein Type
Lysate
Amino Acid Sequence
1MAPDLASQRH SESFPSVNSR PNVILPGREG RREGLPPGGG TRGSLVPTRP VPPSPAPLGT
61SPYSWSRSGP GRGGGAGSSR VPRGVPGPAV CAPGSLLHHA SPTQTMAAAD GSLFDNPRTF
121SRRPPAQASR QAKATKRKYQ ASSEAPPAKR RNETSFLPAK KTSVKETQRT FKGNAQKMFS
181PKKHSVSTSD RNQEERQCIK TSSLFKNNPD IPELHRPVVK QVQEKVFTSA AFHELGLHPH
241LISTINTVLK MSSMTSVQKQ SIPVLLEGRD ALVRSQTGSG KTLAYCIPVV QSLQAMESKI
301QRSDGPYALV LVPTRELALQ SFDTVQKLLK PFTWIVPGVL MGGEKRKSEK ARLRKGINIL
361ISTPGRLVDH IKSTKNIHFS RLRWLVFDEA DRILDLGFEK DITVILNAVN AECQKRQNVL
421LSATLTEGVT RLADISLHDP VSISVLDKSH DQLNPKDKAV QEVCPPPAGD KLDSFAIPES
481LKQHVTVVPS KLRLVCLAAF ILQKCKFEED QKMVVFFSSC ELVEFHYSLF LQTLLSSSGA
541PASGQLPSAS MRLKFLRLHG GMEQEERTAV FQEFSHSRRG VLLCT
SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV

585 aa + 34 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.