LSBio
Human DCLRE1C / Artemis Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DCLRE1C / Artemis Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DCLRE1C antibodies →
$153.00each
Catalog Number: LS-G83227-20

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Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human Artemis(DCLRE1C) (NM_022487) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
A-SCID | ARTEMIS | DCLRE1C | DCLREC1C | HSNM1C | SCIDA | RS-SCID | SNM1C | SNM1-like protein | Protein A-SCID | Protein artemis | PSO2 homolog | ASCID | DNA cross-link repair 1C | SNM1 homolog C
Origin Species
Human
NCBI GenBank
NM_022487
SwissProt
Q96SD1

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
65.2 kDa
Protein Type
Lysate
Amino Acid Sequence
1MKHQERFLFQ GNNGTVLYTG DFRLAQGEAA RMELLHSGGR VKDIQSVYLD TTFCDPRFYQ
61IPSREECLSG VLELVRSWIT RSPYHVVWLN CKAAYGYEYL FTNLSEELGV QVHVNKLDMF
121RNMPEILHHL TTDRNTQIHA CRHPKAEEYF QWSKLPCGIT SRNRIPLHII SIKPSTMWFG
181ERSRKTNVIV RTGESSYRAC FSFHSSYSEI KDFLSYLCPV NAYPNVIPVG TTMDKVVEIL
241KPLCRSSQST EPKYKPLGKL KRARTVHRDS EEEDDYLFDD PLPIPLRHKV PYPETFHPEV
301FSMTAVSEKQ PEKLRQTPGC CRAECMQSSR FTNFVDCEES NSESEEEVGI PASLQGDLGS
361VLHLQKADGD VPQWEVFFKR NDEITDESLE NFPSSTVAGG SQSPKLFSDS DGESTHISSQ
421NSSQSTHITE QGSQGWDSQS DTVLLSSQER NSGDITSLDK ADYRPTIKEN IPASLMEQNV
481ICPKDTYSDL KSRDKDVTIV PSTGEPTTLS SETHIPEEKS LLNLSTNADS QSSSDFEVPS
541TPEAELPKRE HLQYLYEKLA TGESIAVKKR KCSLLDT
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

577 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.