Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DCLRE1C / Artemis Protein (Over-Expression Lysate Myc-DDK (Flag))
ProteinLSBio Guarantee
| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$504.00each
Catalog Number: LS-G101830-100
Catalog Number: LS-G101830
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human Artemis(DCLRE1C) (NM_022487), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- A-SCID | ARTEMIS | DCLRE1C | DCLREC1C | HSNM1C | SCIDA | RS-SCID | SNM1C | SNM1-like protein | Protein A-SCID | Protein artemis | PSO2 homolog | ASCID | DNA cross-link repair 1C | SNM1 homolog C
- Origin Species
- Human
- NCBI GenBank
- NM_022487
- SwissProt
- Q96SD1
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 65.2 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MKHQERFLFQ GNNGTVLYTG DFRLAQGEAA RMELLHSGGR VKDIQSVYLD TTFCDPRFYQ61IPSREECLSG VLELVRSWIT RSPYHVVWLN CKAAYGYEYL FTNLSEELGV QVHVNKLDMF121RNMPEILHHL TTDRNTQIHA CRHPKAEEYF QWSKLPCGIT SRNRIPLHII SIKPSTMWFG181ERSRKTNVIV RTGESSYRAC FSFHSSYSEI KDFLSYLCPV NAYPNVIPVG TTMDKVVEIL241KPLCRSSQST EPKYKPLGKL KRARTVHRDS EEEDDYLFDD PLPIPLRHKV PYPETFHPEV301FSMTAVSEKQ PEKLRQTPGC CRAECMQSSR FTNFVDCEES NSESEEEVGI PASLQGDLGS361VLHLQKADGD VPQWEVFFKR NDEITDESLE NFPSSTVAGG SQSPKLFSDS DGESTHISSQ421NSSQSTHITE QGSQGWDSQS DTVLLSSQER NSGDITSLDK ADYRPTIKEN IPASLMEQNV481ICPKDTYSDL KSRDKDVTIV PSTGEPTTLS SETHIPEEKS LLNLSTNADS QSSSDFEVPS541TPEAELPKRE HLQYLYEKLA TGESIAVKKR KCSLLDTTRTRPLEQKLISEEDLAANDILDYKDDDDKV
577 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.