LSBio
Human DBF4B / DRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Human DBF4B / DRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))

ProteinLSBio Guarantee
Expression SystemHuman Embryonic Kidney 293T
Format:Lyophilized
See all DBF4B antibodies →
$220.00each
Catalog Number: LS-G91918-20

For bulk orders or custom formulations:

Request a Quote

Overview

Description
This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DBF4B (NM_145663) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
ASKL1 | Dbf4-related factor 1 | DRF1 | Chiffon homolog B | DBF4 homolog B (S. cerevisiae) | Protein DBF4 homolog B | ASK-like protein 1 | CHIFB | DBF4B | ZDBF1B
Origin Species
Human
NCBI GenBank
NM_025104
SwissProt
Q8NFT6

Protein

Expression System
Human Embryonic Kidney 293T
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
67.1 kDa
Protein Type
Lysate
Amino Acid Sequence
1MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG KSFYLDLPAG
61KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS GKSHRGCPSP SPSEVRVETS
121AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ KAIRNQGSIS GGGSGGSSSL LTNARSWGVR
181ILHVDEMMMH VQQLSLASLC VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF
241RPFHHQFKSF PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY
301CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ AGLPRWSGSP
361ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG RAAGQQRWTE SLDGVMGPPA
421SHTCVSATTL LPALPKGSRE QGCLCPCPAS FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE
481NSFAPADIPV KGPLLFPEAR PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR
541LGYLYLLLTQ SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT
601PQPFLHCGFL AVDSG
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

615 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Lyophilized
Formulation
Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4

Storage & Handling

Storage Conditions
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.