Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
DBF4B / DRF1 Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293 |
| Format: | Liquid |
$725.00each
Catalog Number: LS-G106607-100
Catalog Number: LS-G106607
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- Description
- This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DBF4B (NM_145663), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- ASKL1 | Dbf4-related factor 1 | DRF1 | Chiffon homolog B | DBF4 homolog B (S. cerevisiae) | Protein DBF4 homolog B | ASK-like protein 1 | CHIFB | DBF4B | ZDBF1B
- Origin Species
- Human
- NCBI GenBank
- NM_025104
- SwissProt
- Q8NFT6
Protein
- Expression System
- Human Embryonic Kidney 293
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 67.1 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MSEPGKGDDC LELESSMAES RLRAPDLGVS RCLGKCQKNS PGARKHPFSG KSFYLDLPAG61KNLQFLTGAI QQLGGVIEGF LSKEVSYIVS SRREVKAESS GKSHRGCPSP SPSEVRVETS121AMVDPKGSHP RPSRKPVDSV PLSRGKELLQ KAIRNQGSIS GGGSGGSSSL LTNARSWGVR181ILHVDEMMMH VQQLSLASLC VKKQQPKKPE GTCPAAESRT RKVARLKAPF LKIEDESRKF241RPFHHQFKSF PEISFLGPKD ASPFEAPTTL GSMHHTRESK DGEPSPRSAA HTMPRRKKGY301CECCQEAFEE LHVHLQSAQH RSFALEAHLY AEVDRIIAQL SHSFADIPFQ AGLPRWSGSP361ASDCDPLCPE TLHPHQPSHP RAASPRIRKE DSCQASVTQG RAAGQQRWTE SLDGVMGPPA421SHTCVSATTL LPALPKGSRE QGCLCPCPAS FTQSHLVTSL ALLPGEWSPA EDMPLHPSQE481NSFAPADIPV KGPLLFPEAR PWLMSARCWV RPFPFVTWGC LIPHDTTPLH EEVSPCPCLR541LGYLYLLLTQ SLWCRVRVPS LSTAGPIPRT SHPCTLAFPS YLNDHDLGHL CQAKPQGWNT601PQPFLHCGFL AVDSGTRTRPLEQKLISEEDLAANDILDYKDDDDKV
615 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Liquid
- Formulation
- 25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
- Concentration
- 1 mg/mL
Storage & Handling
- Storage Conditions
- Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.