DAGLB Antibody
Catalog Number: LS-C750544
2 sizes / from $281.00
Antibody: Rabbit Polyclonal
Applications: Western Blot
Reactivity: Human
Format: Liquid
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Human DAGLB Protein (Over-Expression Lysate Myc-DDK (Flag))
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| Expression System | Human Embryonic Kidney 293T |
| Format: | Lyophilized |
$153.00each
Catalog Number: LS-G93493-20
Catalog Number: LS-G93493
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- Description
- This product includes lyophilized lysate of HEK293T cells that have been transiently-tranfected with Human DAGLB (NM_001142936) and is intended for use as a Western blot positive control. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4). See related products for a larger volume of this lysate supplied with a vector only control and 2x SDS-Page load buffer, or the purified form of this recombinant protein.
- Category
- Proteins & Peptides > Proteins > Lysates
- Alternative Names
- DAGLB | DAGLBETA | Diacylglycerol lipase | beta | KCCR13L | DGL-beta
- Origin Species
- Human
- SwissProt
- Q8NCG7
Protein
- Expression System
- Human Embryonic Kidney 293T
- Fusion Tag
- c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
- Molecular Weight
- 59.7 kDa
- Protein Type
- Lysate
- Amino Acid Sequence
1MPGMVLFGRR WAIASDDLVF PGFFELVVRV LWWIGILTLY LMHRGKLDCA GGALLSSYLI61VLMILLAVVI CTVSAIMCVS MRGTICNPGP RKSMSKLLYI RLALFFPEMV WASLGAAWVA121DGVQCDRTVV NGIIATVVVR TQIWCPATLR RASPCFISNR TISGTTKSLP RWSAMPQGAP181SCRSRTTDYD LVGGDQLNCH FGSILHTTGL QYRDFIHVSF HDKVYELPFL VALDHRKESV241VVAVRGTMSL QDVLTDLSAE SEVLDVECEV QDRLAHKGIS QAARYVYQRL INDGILSQAF301SIAPEYRLVI VGHSLGGGAA ALLATMLRAA YPQVRCYAFS PPRGLWSKAL QEYSQSFIVS361LVLGKDVIPR LSVTNLEDLK RRILRVVAHC NKPKYKILLH GLWYELFGGN PNNLPTELDG421GDQEVLTQPL LGEQSLLTRW SPAYSFSSDS PLDSSPKYPP LYPPGRIIHL QEEGASGRFG481CCSAAHYSAK WSHEAEFSKI LIGPKMLTDH MPDILMRALD SVVSDRAACV SCPAQGVSSV541DVATRTRPLEQKLISEEDLAANDILDYKDDDDKV
543 aa + 31 aa (cloning site & tags)
Format
- Purity
- Unpurified
- Conjugate/Label
- Unconjugated
- Endotoxin Level
- Not Tested
- Format
- Lyophilized
- Formulation
- Lyophilized. 25mM Tris-HCl, pH 7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProtein inhibitor cocktail mix, 1mM PMSF and 1mM NA3VO4
Storage & Handling
- Storage Conditions
- Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
