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DAGLB Protein (Over-Expression Lysate Myc-DDK (Flag))

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

DAGLB Protein (Over-Expression Lysate Myc-DDK (Flag))

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Expression SystemHuman Embryonic Kidney 293
Format:Liquid
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$504.00each
Catalog Number: LS-G126138-100

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Overview

Description
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human DAGLB (NM_001142936), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4).
Category
Proteins & Peptides > Proteins > Lysates
Alternative Names
DAGLB | DAGLBETA | Diacylglycerol lipase | beta | KCCR13L | DGL-beta
Origin Species
Human
SwissProt
Q8NCG7

Protein

Expression System
Human Embryonic Kidney 293
Fusion Tag
c-Myc Epitope Tag (EQKLISEEDL), FLAG Octapeptide Tag (DYKDDDDK)
Molecular Weight
59.7 kDa
Protein Type
Lysate
Amino Acid Sequence
1MPGMVLFGRR WAIASDDLVF PGFFELVVRV LWWIGILTLY LMHRGKLDCA GGALLSSYLI
61VLMILLAVVI CTVSAIMCVS MRGTICNPGP RKSMSKLLYI RLALFFPEMV WASLGAAWVA
121DGVQCDRTVV NGIIATVVVR TQIWCPATLR RASPCFISNR TISGTTKSLP RWSAMPQGAP
181SCRSRTTDYD LVGGDQLNCH FGSILHTTGL QYRDFIHVSF HDKVYELPFL VALDHRKESV
241VVAVRGTMSL QDVLTDLSAE SEVLDVECEV QDRLAHKGIS QAARYVYQRL INDGILSQAF
301SIAPEYRLVI VGHSLGGGAA ALLATMLRAA YPQVRCYAFS PPRGLWSKAL QEYSQSFIVS
361LVLGKDVIPR LSVTNLEDLK RRILRVVAHC NKPKYKILLH GLWYELFGGN PNNLPTELDG
421GDQEVLTQPL LGEQSLLTRW SPAYSFSSDS PLDSSPKYPP LYPPGRIIHL QEEGASGRFG
481CCSAAHYSAK WSHEAEFSKI LIGPKMLTDH MPDILMRALD SVVSDRAACV SCPAQGVSSV
541DVA
TRTRPLEQKLISEEDLAANDILDYKDDDDKV

543 aa + 31 aa (cloning site & tags)

Format

Purity
Unpurified
Conjugate/Label
Unconjugated
Endotoxin Level
Not Tested
Format
Liquid
Formulation
25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4.
Concentration
1 mg/mL

Storage & Handling

Storage Conditions
Store at -80°C. Avoid freeze-thaw cycles. After incubation with SDS-PAGE sample buffer, SDS-PAGE samples need to be stored in -20°C for long term storage.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.